Different loop arrangements of intramolecular human telomeric (3+1) G-quadruplexes in K + solution Journal Article
| Authors: | Phan, A. T.; Luu, K. N.; Patel, D. J. |
| Article Title: | Different loop arrangements of intramolecular human telomeric (3+1) G-quadruplexes in K + solution |
| Abstract: | Intramolecular G-quadruplexes formed by the human telomeric G-rich strand are promising anticancer targets. Here we show that four-repeat human telomeric DNA sequences can adopt two different intramolecular G-quadruplex folds in K + solution. The two structures contain the (3+1) G-tetrad core, in which three G-tracts are oriented in one direction and the fourth in the opposite direction, with one double-chain-reversal and two edgewise loops, but involve different loop arrangements. This result indicates the robustness of the (3+1) core G-quadruplex topology, thereby suggesting it as an important platform for structure-based drug design. Our data also support the view that multiple human telomeric G-quadruplex conformations coexist in K + solution. Furthermore, even small changes to flanking sequences can perturb the equilibrium between different coexisting G-quadruplex forms. © 2006 Oxford University Press. |
| Keywords: | controlled study; drug targeting; telomere; drug design; dna; guanine; nuclear magnetic resonance spectroscopy; dna flanking region; dna sequence; solutions; high performance liquid chromatography; nucleic acid conformation; protein folding; nucleotide repeat; potassium; nucleic acid structure; nuclear magnetic resonance, biomolecular |
| Journal Title: | Nucleic Acids Research |
| Volume: | 34 |
| Issue: | 19 |
| ISSN: | 0305-1048 |
| Publisher: | Oxford University Press |
| Date Published: | 2006-11-01 |
| Start Page: | 5715 |
| End Page: | 5719 |
| Language: | English |
| DOI: | 10.1093/nar/gkl726 |
| PUBMED: | 17040899 |
| PROVIDER: | scopus |
| PMCID: | PMC1635308 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 93" - "Export Date: 4 June 2012" - "CODEN: NARHA" - "Source: Scopus" |
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