Two-repeat Tetrahymena telomeric d(TGGGGTTGGGGT) sequence interconverts between asymmetric dimeric G-quadruplexes in solution Journal Article
| Authors: | Phan, A. T.; Modi, Y. S.; Patel, D. J. |
| Article Title: | Two-repeat Tetrahymena telomeric d(TGGGGTTGGGGT) sequence interconverts between asymmetric dimeric G-quadruplexes in solution |
| Abstract: | Recently, the two-repeat human telomeric d(TAGGGTTAGGGT) sequence has been shown to form interconverting parallel and antiparallel G-quadruplex structures in solution. Here, we examine the structures formed by the two-repeat Tetrahymena telomeric d(TGGGGTTGGGGT) sequence, which differs from the human sequence only by one G-for-A replacement in each repeat. We show by NMR that this sequence forms two novel G-quadruplex structures in Na+- containing solution. Both structures are asymmetric, dimeric G-quadruplexes involving a core of four stacked G-tetrads and two edgewise loops. The adjacent strands of the G-tetrad core are alternately parallel and antiparallel. All G-tetrads adopt syn·syn·anti·anti alignments, which occur with 5′-syn-anti-syn-anti-3′ alternations along G-tracks. In the first structure (head-to-head), two loops are at one end of the G-tetrad core; in the second structure (head-to-tail), two loops are located on opposite ends of the G-tetrad core. In contrast to the human telomere counterpart, the proportions of the two forms here are similar for a wide range of temperatures; their unfolding rates are also similar, with an activation enthalpy of 153 kJ/mol. © 2004 Elsevier Ltd. All rights reserved. |
| Keywords: | telomere; animals; amino acid substitution; dna; kinetics; guanine; nucleotide sequence; solutions; thermodynamics; nucleic acid conformation; temperature sensitivity; repetitive sequences, nucleic acid; nucleotide repeat; stoichiometry; nuclear magnetic resonance, biomolecular; enthalpy; telomeric dna; priority journal; article; g-quadruplex polymorphism; g-quadruplex unfolding kinetics; interconversion between quadruplex topologies; noe, nuclear overhauser effect; noesy, noe spectroscopy; tetrahymena |
| Journal Title: | Journal of Molecular Biology |
| Volume: | 338 |
| Issue: | 1 |
| ISSN: | 0022-2836 |
| Publisher: | Academic Press Inc., Elsevier Science |
| Date Published: | 2004-04-16 |
| Start Page: | 93 |
| End Page: | 102 |
| Language: | English |
| DOI: | 10.1016/j.jmb.2004.02.042 |
| PROVIDER: | scopus |
| PUBMED: | 15050825 |
| PMCID: | PMC4690524 |
| DOI/URL: | |
| Notes: | J. Mol. Biol. -- Cited By (since 1996):39 -- Export Date: 16 June 2014 -- CODEN: JMOBA -- Source: Scopus |
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