Structure of the human telomere in K(+) solution: A stable basket-type G-quadruplex with only two G-tetrad layers Journal Article
| Authors: | Lim, K. W.; Amrane, S.; Bouaziz, S.; Xu, W.; Mu, Y.; Patel, D. J.; Luu, K. N.; Phan, A. T. |
| Article Title: | Structure of the human telomere in K(+) solution: A stable basket-type G-quadruplex with only two G-tetrad layers |
| Abstract: | Previously, it has been reported that human telomeric DNA sequences could adopt in different experimental conditions four different intramolecular G-quadruplexes each involving three G-tetrad layers, namely, Na(+) solution antiparallel-stranded basket form, K(+) crystal parallel-stranded propeller form, K(+) solution (3 + 1) Form 1, and K(+) solution (3 + 1) Form 2. Here we present a new intramolecular G-quadruplex adopted by a four-repeat human telomeric sequence in K(+) solution (Form 3). This structure is a basket- type G-quadruplex with only two G-tetrad layers: loops are successively edgewise, diagonal, and edgewise; glycosidic conformations of guanines are syr·syn·anti·anti around each tetrad. Each strand of the core has both a parallel and an antiparallel adjacent strands; there are one narrow, one wide, and two medium grooves. Despite the presence of only two G-tetrads in the core, this structure is more stable than the three-G-tetrad intramolecular G-quadruplexes previously observed for human telomeric sequences in K(+) solution. Detailed structural elucidation of Form 3 revealed extensive base pairing and stacking in the loops capping both ends of the G-tetrad core, which might explain the high stability of the structure. This novel structure highlights the conformational heterogeneity of human telomeric DNA. It establishes a new folding principle for G-quadruplexes and suggests new loop sequences and structures for targeting in human telomeric DNA. © 2009 American Chemical Society. |
| Keywords: | sequence analysis; telomere; genes; dna; nucleic acids; base pairing; dna sequence; conformation; structure analysis; sodium; conformational heterogeneity; experimental conditions; g-quadruplex; g-quadruplexes; high stability; human telomere; human telomeric dna; novel structures; structural elucidation; telomeric sequences; dna sequences; guanine derivative; guanine quadruplex; potassium |
| Journal Title: | Journal of the American Chemical Society |
| Volume: | 131 |
| Issue: | 12 |
| ISSN: | 0002-7863 |
| Publisher: | American Chemical Society |
| Date Published: | 2009-04-01 |
| Start Page: | 4301 |
| End Page: | 4309 |
| Language: | English |
| DOI: | 10.1021/ja807503g |
| PROVIDER: | scopus |
| PMCID: | PMC2662591 |
| PUBMED: | 19271707 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 34" - "Export Date: 30 November 2010" - "CODEN: JACSA" - "Source: Scopus" |
Altmetric
Citation Impact
BMJ Impact Analytics
Related MSK Work