Propeller-type parallel-stranded G-quadruplexes in the human c-myc promoter Journal Article
| Authors: | Phan, A. T.; Modi, Y. S.; Patel, D. J. |
| Article Title: | Propeller-type parallel-stranded G-quadruplexes in the human c-myc promoter |
| Abstract: | The nuclease-hypersensitivity element III1 in the c-myc promoter is a good anticancer target since it largely controls transcriptional activation of the important c-myc oncogene. Recently, the guanine-rich strand of this element has been shown to form an equilibrium between G-quadruplex structures built from two different sets of G-stretches; two models of intramolecular fold-back antiparallel-stranded G-quadruplexes, called "basket" and "chair" forms, were proposed. Here, we show by NMR that two sequences containing these two sets of G-stretches form intramolecular propeller-type parallel-stranded G-quadruplexes in K +-containing solution. The two structures involve a core of three stacked G-tetrads formed by four parallel G-stretches with all anti guanines and three double-chain-reversal loops bridging three G-tetrad layers. The central loop contains two or six residues, while the two other loops contain only one residue. |
| Keywords: | gene targeting; complex formation; genes; transcription initiation; transcription, genetic; dna; guanine; myc protein; genes, myc; gene control; models, genetic; dna sequence; solutions; nucleic acid conformation; oncogene c myc; biochemistry; nuclear magnetic resonance; model; enzymes; g-quadruplexes; guanine derivative; gene structure; potassium ion; nitrogen compounds; activation analysis; promoter regions (genetics); human engineering; humans; article; c-myc promoters; guanines |
| Journal Title: | Journal of the American Chemical Society |
| Volume: | 126 |
| Issue: | 28 |
| ISSN: | 0002-7863 |
| Publisher: | American Chemical Society |
| Date Published: | 2004-07-21 |
| Start Page: | 8710 |
| End Page: | 8716 |
| Language: | English |
| DOI: | 10.1021/ja048805k |
| PROVIDER: | scopus |
| PUBMED: | 15250723 |
| PMCID: | PMC4692381 |
| DOI/URL: | |
| Notes: | J. Am. Chem. Soc. -- Cited By (since 1996):250 -- Export Date: 16 June 2014 -- CODEN: JACSA -- Source: Scopus |
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