(3 + 1) Assembly of three human telomeric repeats into an asymmetric dimeric G-quadruplex Journal Article
| Authors: | Zhang, N.; Phan, A. T.; Patel, D. J. |
| Article Title: | (3 + 1) Assembly of three human telomeric repeats into an asymmetric dimeric G-quadruplex |
| Abstract: | We present an NMR study on the structure of a DNA fragment of the human telomere containing three guanine-tracts, d(GGGTTAGGGTTAGGGT). This sequence forms in Na+ solution a unique asymmetric dimeric quadruplex, in which the G-tetrad core involves all three G-tracts of one strand and only the last 3′-end G-tract of the other strand. We show that a three-repeat human telomeric sequence can also associate with a single-repeat human telomeric sequence into a structure with the same topology that we name (3 + 1) quadruplex assembly. In this G-quadruplex assembly, there are one syn·syn· syn·anti and two anti· anti·anti·syn G-tetrads, two edgewise loops, three G-tracts oriented in one direction and the fourth oriented in the opposite direction. We discuss the possible implications of the new folding topology for understanding the structure of telomeric DNA, including t-loop formation, and for targeting G-quadruplexes in the telomeres. © 2005 American Chemical Society. |
| Keywords: | sequence analysis; telomere; protein assembly; dna; guanine; magnetic resonance spectroscopy; nuclear magnetic resonance spectroscopy; base sequence; dna sequence; dna structure; models, molecular; dimerization; molecular structure; nucleic acid conformation; nuclear magnetic resonance; nucleotide repeat; human telomere; dna sequences; guanine derivative; dimers; sodium ion; terminal repeat sequences; dna fragments; guanine-tracts |
| Journal Title: | Journal of the American Chemical Society |
| Volume: | 127 |
| Issue: | 49 |
| ISSN: | 0002-7863 |
| Publisher: | American Chemical Society |
| Date Published: | 2005-12-14 |
| Start Page: | 17277 |
| End Page: | 17285 |
| Language: | English |
| DOI: | 10.1021/ja0543090 |
| PUBMED: | 16332077 |
| PROVIDER: | scopus |
| PMCID: | PMC4693638 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 57" - "Export Date: 24 October 2012" - "CODEN: JACSA" - "Source: Scopus" |
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