Detection of lymph node metastases with SERRS nanoparticles Journal Article


Authors: Spaliviero, M.; Harmsen, S.; Huang, R.; Wall, M. A.; Andreou, C.; Eastham, J. A.; Touijer, K. A.; Scardino, P. T.; Kircher, M. F.
Article Title: Detection of lymph node metastases with SERRS nanoparticles
Abstract: Purpose: The accurate detection of lymph node metastases in prostate cancer patients is important to direct treatment decisions. Our goal was to develop an intraoperative imaging approach to distinguish normal from metastasized lymph nodes. We aimed at developing and testing gold-silica surface-enhanced resonance Raman spectroscopy (SERRS) nanoparticles that demonstrate high uptake within normal lymphatic tissue and negligible uptake in areas of metastatic replacement. Procedures: We evaluated the ability of SERRS nanoparticles to delineate lymph node metastases in an orthotopic prostate cancer mouse model using PC-3 cells transduced with mCherry fluorescent protein. Tumor-bearing mice (n = 6) and non-tumor-bearing control animals (n = 4) were injected intravenously with 30 fmol/g SERRS nanoparticles. After 16–18 h, the retroperitoneal lymph nodes were scanned in situ and ex vivo with a Raman imaging system and a handheld Raman scanner and data corroborated with fluorescence imaging for mCherry protein expression and histology. Results: The SERRS nanoparticles demonstrated avid homing to normal lymph nodes, but not to metastasized lymph nodes. In cases where lymph nodes were partially infiltrated by tumor cells, the SERRS signal correctly identified, with sub-millimeter precision, healthy from metastasized components. Conclusions: This study serves as a first proof-of-principle that SERRS nanoparticles enable high precision and rapid intraoperative discrimination between normal and metastasized lymph nodes. © 2016, World Molecular Imaging Society.
Keywords: lymph node metastasis; prostate cancer; intraoperative imaging; raman imaging; surface-enhanced resonance raman scattering
Journal Title: Molecular Imaging and Biology
Volume: 18
Issue: 5
ISSN: 1536-1632
Publisher: Springer  
Date Published: 2016-10-01
Start Page: 677
End Page: 685
Language: English
DOI: 10.1007/s11307-016-0932-2
PROVIDER: scopus
PMCID: PMC5010961
PUBMED: 26943129
DOI/URL:
Notes: Article -- Export Date: 3 October 2016 -- Source: Scopus
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MSK Authors
  1. Peter T Scardino
    671 Scardino
  2. Karim Abdelkrim Touijer
    259 Touijer
  3. Ruimin Huang
    30 Huang
  4. Moritz Florian Kircher
    55 Kircher
  5. James Eastham
    538 Eastham
  6. Stefan Harmsen
    17 Harmsen
  7. Matthew Wall
    10 Wall
  8. Chrysafis   Andreou
    22 Andreou