Tissue factor-specific ultra-bright SERRS nanostars for Raman detection of pulmonary micrometastases Journal Article

   


Authors: Nayak, T. R.; Andreou, C.; Oseledchyk, A.; Marcus, W. D.; Wong, H. C.; Massagué, J.; Kircher, M. F.
Article Title: Tissue factor-specific ultra-bright SERRS nanostars for Raman detection of pulmonary micrometastases
Abstract: Here we demonstrate a novel application of 'surface enhanced resonance Raman scattering nanoparticles' (SERRS NPs) for imaging breast cancer lung metastases with much higher precision than currently feasible. A breast cancer lung metastasis mouse model was established by intravenous injection of LM2 cells. These mice were intravenously administered SERRS NPs conjugated with ALT-836, an anti-tissue factor (TF) monoclonal antibody, and subjected to Raman imaging to visualize the expression of TF both in vivo and ex vivo. Raman imaging indicated marked uptake of αTF-SERRS-NPs by the lung metastases compared to isotype and blocking controls. Conversely, little uptake of αTF-SERRS-NPs was observed in the lungs of healthy mice. Successful detection and delineation of pulmonary micrometastatic lesions as small as 200 μm, corroborated by histology, immunohistochemistry, and bioluminescence imaging confirmed the suitability of both TF as a target and αTF-SERRS-NPs as an effective contrast agent for imaging breast cancer lung metastases. Further advancements of this technique in the form of Raman endoscopes coupled with ultrabright SERRS NPs developed in this work could lead to minimally invasive detection and resection of lung metastases. © 2017 The Royal Society of Chemistry.
Keywords: immunohistochemistry; pathology; monoclonal antibodies; medical imaging; antibodies; mammals; minimally invasive; biological organs; diseases; tissue; bioluminescence imaging; intravenous injections; chemical detection; raman scattering; surface scattering; surface-enhanced resonance raman scattering; breast cancer lung metastasis; effective contrast agents; novel applications
Journal Title: Nanoscale
Volume: 9
Issue: 3
ISSN: 2040-3364
Publisher: Royal Society of Chemistry  
Date Published: 2017-01-21
Start Page: 1110
End Page: 1119
Language: English
DOI: 10.1039/c6nr08217c
PROVIDER: scopus
PUBMED: 27991632
PMCID: PMC5438878
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85010416820&doi=10.1039%2fc6nr08217c&partnerID=40&md5=32647be06a0435f4185acac449482155
Notes: Article -- Export Date: 2 March 2017 -- Source: Scopus
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MSK Authors
  1. Moritz Florian Kircher
    55 Kircher
  2. Joan Massague
    388 Massague
  3. Anton Oseledchyk
    16 Oseledchyk
  4. Chrysafis Andreou
    22 Andreou
  5. Tapas Ranjan Nayak
    1 Nayak
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