| Abstract: |
Chromatin remodeling complexes perform central roles in transcriptional regulation. Here, we identify Rsc3 and Rsc30 as novel components of the essential yeast remodeler RSC complex. Rsc3 and Rsc30 function requires their zinc cluster domain, a known site-specific DNA binding motif. RSC3 is essential, and rsc3 Ts- mutants display a G2/M cell cycle arrest involving the spindle assembly checkpoint pathway, whereas rsc30Δ mutants are viable and osmosensitive. Rsc3 and Rsc30 interact functionally and also physically as a stable Rsc3/Rsc30 heteromeric complex. However, DNA microarray analysis with rsc3 or rsc30 mutants reveals different effects on the expression levels of ribosomal protein genes and cell wall genes. We propose that Rsc3 and Rsc30 interact physically but have different roles in targeting or regulating RSC. |
| Keywords: |
controlled study; unclassified drug; dna binding protein; gene mutation; gene deletion; dna-binding proteins; nonhuman; protein motif; mitosis; protein analysis; cell viability; dna damage; cell cycle; cell growth; protein dna binding; protein protein interaction; protein targeting; protein stability; transcription factors; nuclear proteins; gene expression regulation; cell wall; dna; molecular sequence data; saccharomyces cerevisiae; chromatin; base sequence; binding site; mitotic spindle apparatus; temperature; dimerization; mutagenesis, site-directed; protein structure, tertiary; cell cycle g2 phase; dna microarray; yeast; saccharomyces cerevisiae proteins; zinc; protein structure, secondary; genes, cdc; gene expression regulation, fungal; ribosomal proteins; ribosome protein; g2 phase; genes, lethal; yeasts; dimer; temperature sensitive mutant; article; leucine zippers; protein rsc3; protein rsc30
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