The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation Journal Article

Authors: Hsu, J. M.; Huang, J.; Meluh, P. B.; Laurent, B. C.
Article Title: The yeast RSC chromatin-remodeling complex is required for kinetochore function in chromosome segregation
Abstract: The accurate segregation of chromosomes requires the kinetochore, a complex protein machine that assembles onto centromeric DNA to mediate attachment of replicated sister chromatids to the mitotic spindle apparatus. This study reveals an important role for the yeast RSC ATP-dependent chromatin-remodeling complex at the kinetochore in chromosome transmission. Mutations in genes encoding two core subunits of RSC, the ATPase Sth1p and the Snf5p homolog Sfh1p, interact genetically with mutations in genes encoding kinetochore proteins and with a mutation in centromeric DNA. RSC also interacts genetically and physically with the histone and histone variant components of centromeric chromatin. Importantly, RSC is localized to centromeric and centromere-proximal chromosomal regions, and its association with these loci is dependent on Sth1p. Both sth1 and sfh1 mutants exhibit altered centromeric and centromere-proximal chromatin structure and increased missegregation of authentic chromosomes. Finally, RSC is not required for centromeric deposition of the histone H3 variant Cse4p, suggesting that RSC plays a role in reconfiguring centromeric and flanking nucleosomes following Cse4p recruitment for proper chromosome transmission.
Keywords: unclassified drug; gene mutation; mutation; dna-binding proteins; nonhuman; protein localization; mitosis; chromosome; cell cycle proteins; chromosomal proteins, non-histone; protein; membrane proteins; gene locus; transcription factors; nuclear proteins; dna; saccharomyces cerevisiae; histone; chromatin; histone h3; carrier proteins; mitotic spindle apparatus; yeast; saccharomyces cerevisiae proteins; sequence homology; histones; centromere; chromosome segregation; chromatin structure; macromolecular substances; nucleosome; kinetochores; priority journal; article; phospholipid transfer proteins; protein sfh1p; protein snf5p; protein sth1p
Journal Title: Molecular and Cellular Biology
Volume: 23
Issue: 9
ISSN: 0270-7306
Publisher: American Society for Microbiology  
Date Published: 2003-05-01
Start Page: 3202
End Page: 3215
Language: English
DOI: 10.1128/mcb.23.9.3202-3215.2003
PUBMED: 12697820
PROVIDER: scopus
PMCID: PMC153182
Notes: Export Date: 12 September 2014 -- Source: Scopus
Citation Impact
MSK Authors
  1. Pamela Meluh
    8 Meluh