The genome-wide localization of Rsc9, a component of the RSC chromatin-remodeling complex, changes in response to stress Journal Article
| Authors: | Damelin, M.; Simon, I.; Moy, T. I.; Wilson, B.; Komili, S.; Tempst, P.; Roth, F. P.; Young, R. A.; Cairns, B. R.; Silver, P. A. |
| Article Title: | The genome-wide localization of Rsc9, a component of the RSC chromatin-remodeling complex, changes in response to stress |
| Abstract: | The cellular response to environmental changes includes widespread modifications in gene expression. Here we report the identification and characterization of Rsc9, a member of the RSC chromatin-remodeling complex in yeast. The genome-wide localization of Rsc9 indicated a relationship between genes targeted by Rsc9 and genes regulated by stress; treatment with hydrogen peroxide or rapamycin, which inhibits TOR signaling, resulted in genome-wide changes in Rsc9 occupancy. We further show that Rsc9 is involved in both repression and activation of mRNAs regulated by TOR as well as the synthesis of rRNA. Our results illustrate the response of a chromatin-remodeling factor to signaling cascades and suggest that changes in the activity of chromatin-remodeling factors are reflected in changes in their localization in the genome. |
| Keywords: | signal transduction; controlled study; unclassified drug; dna-binding proteins; nonhuman; flow cytometry; protein localization; protein analysis; animals; oxidants; protein; transcription factors; nuclear proteins; amino acid sequence; molecular sequence data; messenger rna; rna synthesis; sequence alignment; chromatin; phosphotransferases (alcohol group acceptor); 1-phosphatidylinositol 3-kinase; gene control; stress; genome; hydrogen peroxide; yeast; saccharomyces cerevisiae proteins; ribosome rna; cell separation; rapamycin; sirolimus; gene expression regulation, fungal; northern blotting; sequence analysis, dna; genome, fungal; yeasts; fungal proteins; antibiotics, antifungal; article; protein rsc9 |
| Journal Title: | Molecular Cell |
| Volume: | 9 |
| Issue: | 3 |
| ISSN: | 1097-2765 |
| Publisher: | Cell Press |
| Date Published: | 2002-03-01 |
| Start Page: | 563 |
| End Page: | 573 |
| Language: | English |
| DOI: | 10.1016/s1097-2765(02)00475-6 |
| PUBMED: | 11931764 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
