Two actin-related proteins are shared functional components of the chromatin-remodeling complexes RSC and SWI/SNF Journal Article

Authors: Cairns, B. R.; Erdjument-Bromage, H.; Tempst, P.; Winston, F.; Kornberg, R. D.
Article Title: Two actin-related proteins are shared functional components of the chromatin-remodeling complexes RSC and SWI/SNF
Abstract: The yeast Saccharomyces cerevisiae contains two related chromatin-remodeling complexes, RSC and SWI/ SNF, which are shown to share the actin-related proteins Arp7 and Arp9. Depending on the genetic background tested, arp7Δ and arp9Δ mutants are either inviable or show greatly impaired growth and Swi-/ Snf- mutant phenotypes. Unlike swi/snf mutants, viable arp7Δ or arp9Δ mutants have an Spt- phenotype, suggesting that RSC affects transcription. Temperature-sensitive mutations in ARP7and ARP9 were isolated, and the amino acid changes support the structural relationship of Arp7 and Arp9 to actin. However, site-directed mutations predicted to impair ATP binding or hydrolysis did not detectably affect Arp7 or Arp9 function. Our results suggest that actin-related proteins perform important roles in chromatin-remodeling complexes by virtue of structural rather than enzymatic similarities to actin.
Keywords: carrier protein; dna binding protein; genetics; dna-binding proteins; molecular genetics; nonhistone protein; phenotype; metabolism; chromosomal proteins, non-histone; actin; cell division; amino acid substitution; protein binding; transcription factor; genetic transcription; transcription, genetic; transcription factors; chemistry; amino acid sequence; conserved sequence; molecular sequence data; hybrid protein; recombinant fusion proteins; saccharomyces cerevisiae; chromatin; nucleotide sequence; carrier proteins; mutagenesis, site-directed; heat shock protein 70; saccharomyces cerevisiae proteins; adenosine triphosphate; saccharomyces cerevisiae protein; protein secondary structure; actins; adenosine triphosphatase; site directed mutagenesis; isolation and purification; adenosine triphosphatases; protein structure, secondary; fungal protein; hsp70 heat-shock proteins; fungal proteins; rsc complex, s cerevisiae; hsc70 heat-shock proteins; article; arp7 protein, s cerevisiae; arp9 protein, s cerevisiae; heat shock cognate protein 70; swi1 protein, s cerevisiae
Journal Title: Molecular Cell
Volume: 2
Issue: 5
ISSN: 1097-2765
Publisher: Cell Press  
Date Published: 1998-11-01
Start Page: 639
End Page: 651
Language: English
PUBMED: 9844636
PROVIDER: scopus
DOI: 10.1016/S1097-2765(00)80162-8
Notes: Article -- Export Date: 12 December 2016 -- Source: Scopus
Citation Impact
MSK Authors
  1. Paul J Tempst
    323 Tempst