RSC, an essential, abundant chromatin-remodeling complex Journal Article


Authors: Cairns, B. R.; Lorch, Y.; Li, Y.; Zhang, M.; Lacomis, L.; Erdjument-Bromage, H.; Tempst, P.; Du, J.; Laurent, B.; Kornberg, R. D.
Article Title: RSC, an essential, abundant chromatin-remodeling complex
Abstract: A novel 15-subunit complex with the capacity to remodel the structure of chromatin, termed RSC, has been isolated from S. cerevisiae on the basis of homology to the SWI/SNF complex. At least three RSC subunits are related to SWI/SNF polypeptides: Sth1p, Rsc6p, and Rsc8p are significantly similar to Swi2/Snf2p, Swp73p, and Swi3p, respectively, and were identified by mass spectrometric and sequence analysis of peptide fragments. Like SWI/SNF, RSC exhibits a DNA-dependent ATPase activity stimulated by both free and nucleosomal DNA and a capacity to perturb nucleosome structure. RSC is, however, at least 10-fold more abundant than SWI/SNF complex and is essential for mitotic growth. Contrary to a report for SWI/SNF complex, no association of RSC (nor of SWI/SNF complex) with RNA polymerase II holoenzyme was detected.
Keywords: sequence analysis; protein analysis; protein binding; protein purification; saccharomyces cerevisiae; structure analysis; adenosine triphosphatase; enzyme substrate complex; rna polymerase ii; chromatin structure; dna directed dna polymerase; priority journal; article
Journal Title: Cell
Volume: 87
Issue: 7
ISSN: 0092-8674
Publisher: Cell Press  
Date Published: 1996-12-27
Start Page: 1249
End Page: 1260
Language: English
DOI: 10.1016/s0092-8674(00)81820-6
PUBMED: 8980231
PROVIDER: scopus
DOI/URL:
Notes: Article -- Export Date: 22 November 2017 -- Source: Scopus
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MSK Authors
  1. Paul J Tempst
    323 Tempst
  2. Lynne M Lacomis
    21 Lacomis