RSC, an essential, abundant chromatin-remodeling complex Journal Article
| Authors: | Cairns, B. R.; Lorch, Y.; Li, Y.; Zhang, M.; Lacomis, L.; Erdjument-Bromage, H.; Tempst, P.; Du, J.; Laurent, B.; Kornberg, R. D. |
| Article Title: | RSC, an essential, abundant chromatin-remodeling complex |
| Abstract: | A novel 15-subunit complex with the capacity to remodel the structure of chromatin, termed RSC, has been isolated from S. cerevisiae on the basis of homology to the SWI/SNF complex. At least three RSC subunits are related to SWI/SNF polypeptides: Sth1p, Rsc6p, and Rsc8p are significantly similar to Swi2/Snf2p, Swp73p, and Swi3p, respectively, and were identified by mass spectrometric and sequence analysis of peptide fragments. Like SWI/SNF, RSC exhibits a DNA-dependent ATPase activity stimulated by both free and nucleosomal DNA and a capacity to perturb nucleosome structure. RSC is, however, at least 10-fold more abundant than SWI/SNF complex and is essential for mitotic growth. Contrary to a report for SWI/SNF complex, no association of RSC (nor of SWI/SNF complex) with RNA polymerase II holoenzyme was detected. |
| Keywords: | sequence analysis; protein analysis; protein binding; protein purification; saccharomyces cerevisiae; structure analysis; adenosine triphosphatase; enzyme substrate complex; rna polymerase ii; chromatin structure; dna directed dna polymerase; priority journal; article |
| Journal Title: | Cell |
| Volume: | 87 |
| Issue: | 7 |
| ISSN: | 0092-8674 |
| Publisher: | Cell Press |
| Date Published: | 1996-12-27 |
| Start Page: | 1249 |
| End Page: | 1260 |
| Language: | English |
| DOI: | 10.1016/s0092-8674(00)81820-6 |
| PUBMED: | 8980231 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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