Tandem bromodomains in the chromatin remodeler RSC recognize acetylated histone H3 Lys14 Journal Article
| Authors: | Kasten, M.; Szerlong, H.; Erdjument-Bromage, H.; Tempst, P.; Werner, M.; Cairns, B. R. |
| Article Title: | Tandem bromodomains in the chromatin remodeler RSC recognize acetylated histone H3 Lys14 |
| Abstract: | The coordination of chromatin remodeling with chromatin modification is a central topic in gene regulation. The yeast chromatin remodeling complex RSC bears multiple bromodomains, motifs for acetyl-lysine and histone tail interaction. Here, we identify and characterize Rsc4 and show that it bears tandem essential bromodomains. Conditional rsc4 bromodomain mutations were isolated, and were lethal in combination with gcn5Δ, whereas combinations with esa1 grew well. Replacements involving Lys14 of histone H3 (the main target of Gcn5), but not other H3 or H4 lysine residues, also conferred severe growth defects to rsc4 mutant strains. Importantly, wild-type Rsc4 bound an H3 tail peptide acetylated at Lys14, whereas a bromodomain mutant derivative did not. Loss of particular histone deacetylases suppressed rsc4 bromodomain mutations, suggesting that Rsc4 promotes gene activation. Furthermore, rsc4 mutants displayed defects in the activation of genes involved in nicotinic acid biosynthesis, cell wall integrity, and other pathways. Taken together, Rsc4 bears essential tandem bromodomains that rely on H3 Lys14 acetylation to assist RSC complex for gene activation. |
| Keywords: | controlled study; gene mutation; promoter region; gene deletion; dna-binding proteins; nonhuman; protein domain; protein analysis; gene targeting; protein kinases; protein binding; transcription, genetic; wild type; transcription factors; gene activation; biosynthesis; cell wall; disease severity; amino acid sequence; molecular sequence data; saccharomyces cerevisiae; sequence alignment; molecular recognition; histone h3; mutagenesis, site-directed; protein structure, tertiary; saccharomyces cerevisiae proteins; repressor proteins; histones; histone deacetylases; lysine; chromatin assembly and disassembly; mutant; growth disorder; acetylation; nicotinic acid; enzyme repression; rsc; bromodomain; gene isolation; histone acetyltransferases; histone acetylation; chromatin remodeling; priority journal; article; genetic strain |
| Journal Title: | EMBO Journal |
| Volume: | 23 |
| Issue: | 6 |
| ISSN: | 0261-4189 |
| Publisher: | Wiley Blackwell |
| Date Published: | 2004-03-24 |
| Start Page: | 1348 |
| End Page: | 1359 |
| Language: | English |
| DOI: | 10.1038/sj.emboj.7600143 |
| PROVIDER: | scopus |
| PMCID: | PMC381415 |
| PUBMED: | 15014446 |
| DOI/URL: | |
| Notes: | EMBO J. -- Cited By (since 1996):123 -- Export Date: 16 June 2014 -- CODEN: EMJOD -- Source: Scopus |
