Novel 2D and 3D multiple-quantum bi-directional HCNCH experiments for the correlation of ribose and base protons/carbons in 13C/15N labeled RNA Journal Article

  

Authors:	Hu, W.; Gosser, Y. Q.; Xu, W.; Patel, D. J.
Article Title:	Novel 2D and 3D multiple-quantum bi-directional HCNCH experiments for the correlation of ribose and base protons/carbons in 13C/15N labeled RNA
Abstract:	Multiple-quantum 2D and 3D bi-directional HCNCH experiments are presented for the correlation of base and ribose protons/carbons in 13C/15N labeled HIV-1 TAR RNA. In both 2D and 3D experiments, the magnetization of H1′ is transferred to H6/H8 and H1′-C1′-N1/9-C6/8-H6/8 and H1′-C1′-N1/9-C1′-H1′ pathways, and the magnetization of H6/8 is transferred to H1′ and H6/8 through H6/8-C6/8-N1/9-C1′-H1′ and H6/8-C6/8/-N1/9-C6/8-H6/8 pathways. Chemical shifts of four different nuclei (H1′, C1′, C6/8 and H6/8) are sampled in the 2D experiment. The correlation of base and ribose protons/carbons is established by the rectangular arrangement of crossover and out-and-back peaks in the proton/carbon correlated spectrum. The rectangular connections can be further resolved using the nitrogen dimension in a 1H/13C/15N 3D experiment. Furthermore, by taking advantage of the well separated chemical shifts of N1 (pyrimidine) and N9 (purine), the 2D spectrum can be simplified into two sub-spectra based on their base type. Both experiments were tested on a 13C/15N labeled 27-mer HIV-1 TAR RNA containing a UUCG hairpin loop.
Keywords:	controlled study; rna; guanine; virus rna; three dimensional imaging; nuclear magnetic resonance spectroscopy; protons; human immunodeficiency virus; nitrogen; rna, viral; proton nuclear magnetic resonance; human immunodeficiency virus 1; pyrimidine; sample; spectrum; carbon; proton; carbon isotopes; nitrogen isotopes; carbon nuclear magnetic resonance; purine; nuclear magnetic resonance, biomolecular; uracil; cytosine; quantum mechanics; ribose; carbon 13; hiv long terminal repeat; experiment; priority journal; article; base-sugar proton/carbon correlation; bi-directional hcnch; multiple-quantum coherence; rna oligomers
Journal Title:	Journal of Biomolecular NMR
Volume:	20
Issue:	2
ISSN:	0925-2738
Publisher:	Springer
Date Published:	2001-06-01
Start Page:	167
End Page:	172
Language:	English
DOI:	10.1023/a:1011226415824
PUBMED:	11495247
PROVIDER:	scopus
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-0034924753&partnerID=40&md5=e5e81b76494e5091c9e4e8f5e3fb81ab
Notes:	Export Date: 21 May 2015 -- Source: Scopus

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