Sensitivity-enhanced MQ-HCN-CCH-TOCSY and MQ-HCN-CCH-COSY pulse schemes for 13C/15N labeled RNA oligonucleotides Journal Article

  


Authors: Hu, W.; Jiang, L.; Gosser, Y. Q.
Article Title: Sensitivity-enhanced MQ-HCN-CCH-TOCSY and MQ-HCN-CCH-COSY pulse schemes for 13C/15N labeled RNA oligonucleotides
Abstract: Sensitivity enhanced multiple-quantum 3D HCN-CCH-TOCSY and HCN-CCH-COSY experiments are presented for the ribose resonance assignment of 13C/15N-labeled RNA sample. The experiments make use of the chemical shift dispersion of N1/N9 of pyrimidine/purine to distinguish the ribose spin systems. They provide a complementary approach for the assignment of ribose resonance to the currently used HCCH-COSY and HCCH-TOCSY type experiments in which either 13C or 1H is utilized to separate the different ribose spin systems. The pulse schemes have been demonstrated on a 23-mer 13C/15N-labeled RNA aptamer complexed with neomycin and tested on a 32-mer RNA complexed with a 23-residue peptide. © 2000 Academic Press.
Keywords: methodology; sensitivity and specificity; rna; chemistry; image enhancement; nuclear magnetic resonance; nitrogen; carbon; carbon isotopes; nitrogen isotopes; nuclear magnetic resonance, biomolecular; oligoribonucleotide; oligoribonucleotides; article; assignment; multiple-quantum coherence; sensitivity enhancement; hcn-cch-cosy; hcn-cch-tocsy
Journal Title: Journal of Magnetic Resonance
Volume: 145
Issue: 1
ISSN: 1090-7807
Publisher: Academic Press Inc., Elsevier Science  
Date Published: 2000-07-01
Start Page: 147
End Page: 151
Language: English
DOI: 10.1006/jmre.2000.2103
PUBMED: 10873506
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-0034219270&partnerID=40&md5=b13ef25602d8d553319b31bf146de49c
Notes: Export Date: 18 November 2015 -- Source: Scopus
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MSK Authors
  1. Yuying Gosser
    7 Gosser
  2. Weidong Hu
    16 Hu
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