Authors: | Majumdar, A.; Gosser, Y.; Patel, D. J. |
Article Title: | 1H-1H correlations across N-H•••N hydrogen bonds in nucleic acids |
Abstract: | In 2HJNN-COSY experiments, which correlate protons with donor/acceptor nitrogens across Nd•••HNa bonds, the receptor nitrogen needs to be assigned in order to unambiguously identify the hydrogen bond. For many situations this is a non-trivial task which is further complicated by poor dispersion of (Na,Nd) resonances. To address these problems, we present pulse sequences to obtain direct, internucleotide correlations between protons in uniformly 13C/15N labeled nucleic acids containing Nd•••HNa hydrogen bonds. Specifically, the pulse sequence H2(N1N3)H3 correlates H2(A,ω1):H3(U,ω2) protons across Watson-Crick A-U and mismatched G•A base pairs, the sequences H5(N3N1)H1/H6(N3N1)H1 correlate need for independent assignment of the donor/acceptor nitrogen and related degeneracy issues associated with poorly dispersed nitrogen resonances. The methodology is demonstrated on uniformly 13C/15N labeled samples of (a) an RNA regulatory element involving the HIV-1 TAR RNA fragment, (b) a multi-stranded DNA architecture involving a G•(C-A) triad-containing G-quadruplex and (c) a peptide-RNA complex involving an evolved peptide bound to the HIV-1 Rev response element (RRE) RNA fragment. |
Keywords: | controlled study; genetics; methodology; metabolism; peptide; structure activity relation; structure-activity relationship; rna; chemistry; dna; guanine; virus rna; nucleotide sequence; nucleic acids; peptides; nuclear magnetic resonance spectroscopy; base sequence; base pairing; hydrogen bond; hydrogen bonding; models, molecular; protons; chemical structure; human immunodeficiency virus; conformation; nucleic acid conformation; dna helix; nuclear magnetic resonance; nitrogen; rna, viral; human immunodeficiency virus 1; sample; hiv-1; proton; nucleotide; long terminal repeat; nucleic acid; adenine; protein rna binding; hydrogen; nuclear magnetic resonance, biomolecular; base mispairing; base pair mismatch; uracil; carbon 13; dispersion; hiv long terminal repeat; quadruplex dna; humans; human; priority journal; article; rev protein; 2hjnn; hnn-cosy; h2(n1n3)h3; h5(n3n1)h1; h6(n3n1)h1; h2(n2n7)h8; gene products, rev |
Journal Title: | Journal of Biomolecular NMR |
Volume: | 21 |
Issue: | 4 |
ISSN: | 0925-2738 |
Publisher: | Springer |
Date Published: | 2001-12-01 |
Start Page: | 289 |
End Page: | 306 |
Language: | English |
DOI: | 10.1023/a:1013340227140 |
PUBMED: | 11824750 |
PROVIDER: | scopus |
DOI/URL: | |
Notes: | Export Date: 21 May 2015 -- Source: Scopus |