Observation of internucleotide NH···N hydrogen bonds in the absence of directly detectable protons Journal Article
| Authors: | Majumdar, A.; Kettani, A.; Skripkin, E.; Patel, D. J. |
| Article Title: | Observation of internucleotide NH···N hydrogen bonds in the absence of directly detectable protons |
| Abstract: | Several structural motifs found in nucleic acids involve N-H···N hydrogen bonds in which the donor hydrogens are broadened to extinction due to chemical or conformational exchange. In such situations, it is impossible to use the well-established HNN-COSY or soft HNN-COSY experiments, which report the presence of the hydrogen bond directly on the donor proton(s). We present a pulse sequence, H(CN)N(H), for alleviating this problem in hydrogen bonds of the type N(d)H···N(a)-CH, in which the donor N(d) nitrogen is correlated with the corresponding non-exchangeable C-H proton associated with the acceptor N(a) nitrogen. In this way, missing N(d)H···N(a) correlations in an HNN-COSY spectrum may be recovered from CH-N(d) correlations in the H(CN)N(H) spectrum. By correlating a different set of nuclei relative to the HNN-COSY class of experiments, the H(CN)N(H) experiment also serves to remove ambiguities associated with degeneracies in HNN-COSY spectra. The technique is demonstrated on d(GGAGGAG)(4),a quadruplex containing a novel A·(G·G·G·G) · A hexad and on d(GGGCAGGT)(4), containing a G·C·G·C tetrad, in which missing NH2···N7 correlations are retrieved via H8- (N2,N6) correlations in the H(CN)N(H) spectrum. |
| Keywords: | nuclear magnetic resonance imaging; guanine; base pairing; hydrogen bond; hydrogen bonding; protons; conformational transition; nucleic acid conformation; protein folding; protein determination; spectroscopy; proton transport; nitrogen; adenine; nucleic acid structure; hydrogen; nuclear magnetic resonance, biomolecular; priority journal; article; a·(g·g·g·g)·a hexad; (2h)j(nn); g·c·g·c tetrad; g·g·g tetrad; hnn- cosy |
| Journal Title: | Journal of Biomolecular NMR |
| Volume: | 15 |
| Issue: | 3 |
| ISSN: | 0925-2738 |
| Publisher: | Springer |
| Date Published: | 1999-11-01 |
| Start Page: | 207 |
| End Page: | 211 |
| Language: | English |
| DOI: | 10.1023/a:1008357304708 |
| PUBMED: | 10677824 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
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