Observation and measurement of internucleotide 2J(NN) coupling constants between 15N nuclei with widely separated chemical shifts Journal Article
| Authors: | Majumdar, A.; Kettani, A.; Skripkin, E. |
| Article Title: | Observation and measurement of internucleotide 2J(NN) coupling constants between 15N nuclei with widely separated chemical shifts |
| Abstract: | Scalar coupling correlations between hydrogen bonded 15N nuclei in non Watson-Crick base pairs is a critical step in the structure determination of unusual nucleic acids. For observing the 2J(NN) coupling constant between far upfield N2,N6 (amino) nitrogens and far downfield (N1,N3,N7) nitrogens (separated by 150-160 ppm), the HNNCOSY experiment (Dingley and Grzesiek, 1998) is rather insensitive, due to technical difficulties associated with simultaneous excitation of both extremes of the 15N spectrum. These nuclei may be correlated by treating them in a pseudo-heteronuclear manner, using 15N selective pulses. The wide chemical shift separation allows accurate measurement of the 2J(NN) coupling constant using spin-echo difference methods. Pulse sequences for observation and measurement of 2J(NN) coupling constants between amino and N7 nuclei are presented and demonstrated on an A- A mismatch segment of the uniformly (15N, 13C) labelled DNA sample, d(GGAGGAT)2. |
| Keywords: | sequence analysis; protein analysis; dna; base sequence; base pairing; dna sequence; hydrogen bond; hydrogen bonding; nucleic acid conformation; protein structure; structure analysis; nuclear magnetic resonance; nucleic acid; oligodeoxyribonucleotides; nuclear magnetic resonance, biomolecular; priority journal; article; hnn-cosy; 2j(nn), spin-echo difference; internucleotide 15n couplings |
| Journal Title: | Journal of Biomolecular NMR |
| Volume: | 14 |
| Issue: | 1 |
| ISSN: | 0925-2738 |
| Publisher: | Springer |
| Date Published: | 1999-05-01 |
| Start Page: | 67 |
| End Page: | 70 |
| Language: | English |
| DOI: | 10.1023/a:1008335502416 |
| PUBMED: | 10382306 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
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