Determination of (3)J(H3(′)(i), P(i+1)) and (3)J(H5(′)(i)/5(″)(i), P(i)) coupling constants in (13)C-labeled nucleic acids using constant-time HMQC Journal Article
| Authors: | Hu, W.; Bouaziz, S.; Skripkin, E.; Kettani, A. |
| Article Title: | Determination of (3)J(H3(′)(i), P(i+1)) and (3)J(H5(′)(i)/5(″)(i), P(i)) coupling constants in (13)C-labeled nucleic acids using constant-time HMQC |
| Abstract: | A novel 1H-13C correlated two-dimensional experiment, CT-HMQC-J, for the measurement of three-bond proton-phosphorus coupling constants in 13C-labeled DNA is described. The experiment is based on the intensity difference of 1H-13C cross peaks in the presence and absence of the proton-phosphorus coupling interaction during the constant-time period in HMQC experiment. The 3J(H, P) coupling constants can be easily extracted from the intensity ratios of the two experiments. The method has been applied to a uniformly 13C, 15N-labeled d(GGAGGAT) 7-mer DNA sample. The proton-phosphorus coupling constants determined from CT-HMQC-J, together with the other three-bond coupling constants, are used to determine β and ∈ torsion angles. The introduction of β and ∈ restraints has improved the convergence as well as the quality of d(GGAGGAT) structure. © 1999 Academic Press. |
| Keywords: | methodology; chemistry; dna; nucleic acids; magnetic resonance spectroscopy; nuclear magnetic resonance spectroscopy; protons; carbon; proton; carbon isotopes; phosphorus; torsion angles; article; 1h-31p coupling constant; ct-hmqc-j; structure refinement |
| Journal Title: | Journal of Magnetic Resonance |
| Volume: | 139 |
| Issue: | 1 |
| ISSN: | 1090-7807 |
| Publisher: | Academic Press Inc., Elsevier Science |
| Date Published: | 1999-07-01 |
| Start Page: | 181 |
| End Page: | 185 |
| Language: | English |
| PUBMED: | 10388597 |
| PROVIDER: | scopus |
| DOI: | 10.1006/jmre.1999.1785 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
Altmetric
Citation Impact
BMJ Impact Analytics
MSK Authors
Related MSK Work