Detection of very weak side chain-main chain hydrogen bonding interactions in medium-size 13C/15N-labeled proteins by sensitivity- enhanced NMR spectroscopy Journal Article

  


Authors: Liu, A.; Hu, W.; Majumdar, A.; Rosen, M. K.; Patel, D. J.
Article Title: Detection of very weak side chain-main chain hydrogen bonding interactions in medium-size 13C/15N-labeled proteins by sensitivity- enhanced NMR spectroscopy
Abstract: We describe the direct observation of very weak side chain-main chain hydrogen bonding interactions in medium-size 13C/15N-labeled proteins with sensitivity-enhanced NMR spectroscopy. Specifically, the remote correlation between the hydrogen acceptor side chain carboxylate carbon 13CO2(δ) of glutamate 54 and the hydrogen donor backbone amide 15N of methionine 49 in a 12 kDa protein, human FKBP12, is detected via the trans- hydrogen bond (3h)J(NCO2δ) coupling by employing a novel sensitivity- enhanced HNCO-type experiment, CPD-HNCO. The (3h)J(NCO2δ) coupling constant appears to be even smaller than the average value of backbone (3h)J(NC') couplings, consistent with more extensive local dynamics in protein side chains.
Keywords: molecular dynamics; image enhancement; nuclear magnetic resonance spectroscopy; hydrogen bond; hydrogen bonding; molecular interaction; protein structure; m protein; glutamic acid; signal detection; methionine; nitrogen; carbon isotopes; nitrogen isotopes; nuclear magnetic resonance, biomolecular; molecular size; carbon 13; humans; priority journal; article; tacrolimus binding protein 1a; fkbp12; sensitivity enhancement; nmr detection; side chain-main chain hydrogen bonds
Journal Title: Journal of Biomolecular NMR
Volume: 17
Issue: 1
ISSN: 0925-2738
Publisher: Springer  
Date Published: 2000-05-01
Start Page: 79
End Page: 82
Language: English
DOI: 10.1023/a:1008373501591
PUBMED: 10909868
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-0033936896&partnerID=40&md5=3fd6fb70bcc44ba9a2858b8949bb73b6
Notes: Export Date: 18 November 2015 -- Source: Scopus
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MSK Authors
  1. Michael Rosen
    19 Rosen
  2. Weidong Hu
    16 Hu
  3. Ananya Majumdar
    33 Majumdar
  4. Dinshaw J Patel
    478 Patel
  5. Aizhuo Liu
    5 Liu
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