Multiple-quantum HCN-CCH-TOCSY experiment for 13C/15N labeled RNA oligonucleotides Journal Article
| Authors: | Hu, W.; Jiang, L. |
| Article Title: | Multiple-quantum HCN-CCH-TOCSY experiment for 13C/15N labeled RNA oligonucleotides |
| Abstract: | A multiple-quantum 3D HCN-CCH-TOCSY experiment is presented for the assignment of RNA ribose resonances. The experiment makes use of the chemical shift dispersion of N1 of pyrimidine and N9 of purine to distinguish the ribose spin systems. It provides an alternative approach for the assignment of ribose resonances to the currently used COSY- and TOCSY-type experiments in which either 13C or 1H is utilized to distinguish the different spin systems. Compared to the single-quantum version, the sensitivity of the multiple-quantum HCN-CCH-TOCSY experiment is enhanced on average by a factor of 2 for a 23-mer RNA aptamer complexed with neomycin. |
| Keywords: | sensitivity and specificity; rna; three dimensional imaging; nuclear magnetic resonance spectroscopy; rna structure; structure analysis; proton nuclear magnetic resonance; carbon nuclear magnetic resonance; nuclear magnetic resonance, biomolecular; rna analysis; quantum theory; quantum chemistry; ribose; oligoribonucleotides; priority journal; article; assignment; multiple-quantum coherence; hcn-cch-tocsy; framycetin |
| Journal Title: | Journal of Biomolecular NMR |
| Volume: | 15 |
| Issue: | 4 |
| ISSN: | 0925-2738 |
| Publisher: | Springer |
| Date Published: | 1999-12-01 |
| Start Page: | 289 |
| End Page: | 293 |
| Language: | English |
| DOI: | 10.1023/a:1008391007013 |
| PUBMED: | 10685339 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 16 August 2016 -- Source: Scopus |
