A new member of the NY-ESO-1 gene family is ubiquitously expressed in somatic tissues and evolutionarily conserved Journal Article
| Authors: | Alpen, B.; Güre, A. O.; Scanlan, M. J.; Old, L. J.; Chen, Y. T. |
| Article Title: | A new member of the NY-ESO-1 gene family is ubiquitously expressed in somatic tissues and evolutionarily conserved |
| Abstract: | The NY-ESO-1 gene, located on chromosome Xq28, encodes a cancer/testis antigen in human. Normally expressed only in germ cells, NY-ESO-1 is activated in a wide range of human tumors, eliciting both antibody and cell-mediated immune responses in cancer patients. Clinical immunotherapeutic trials NY-ESO-1 gene products are now ongoing. A closely related gene, LAGE-1, was subsequently identified and shares similar biological features. By database search, we have identified a third member of the human NY-ESO-1 gene family. This gene, designated ESO3, is also located on the X chromosome, clustered with two exact copies of ESO1(NY-ESO-1) and one copy of ESO2(LAGE-1), within a ∼400 kb segment. The exon-intron structures are conserved among ESO1-3. While ESO1 and ESO2LAGE-1 share >80% protein sequence identity, homology between ESO3 and the other two members is lower (<50%). ESO3 is also distinctive in that, unlike ESO1 and 2 that are normally expressed only in testis, ESO3 messenger RNA (mRNA) is ubiquitously expressed in somatic tissues. In addition to ESO3, an intronless pseudogene highly homologous to ESO3 was found on chromosome 9, designated ψESO3. A search of the rodent databases identified mouse and rat counterparts of ESO3, named mESO3 and rESO3. mESO3 is similarly located on mouse X chromosome, with conserved exon-intron junctions. Protein sequence of mESO3 is 54% identical to ESO3 (70% identical at the conserved carboxyl end), and 32% to ESO1. mESO3 mRNA is also ubiquitously expressed in somatic tissues, as is rESO3. In addition, an intronless and presumably non-coding, copy of the mESO3, ψmESO3, was identified on mouse chromosome 15. No counterpart of the ESO1 or 2 genes was found in rodents. © 2002 Elsevier Science B.V. All rights reserved. |
| Keywords: | controlled study; human tissue; unclassified drug; exon; nonhuman; proteins; mouse; animals; mice; animal tissue; gene expression; protein; membrane proteins; intron; gene product; data base; evolution, molecular; molecular cloning; amino acid sequence; conserved sequence; molecular sequence data; sequence homology, amino acid; immunotherapy; antigens, neoplasm; cancer vaccine; messenger rna; reverse transcriptase polymerase chain reaction; rna, messenger; gene identification; nucleotide sequence; rat; alternative splicing; x chromosome; base sequence; rodent; rodentia; cancer/testis antigen; sequence homology; testis; genetic conservation; gene location; antigens, surface; gene structure; multigene family; chromosome 9; pseudogene; pseudogenes; protein ny eso 1; humans; human; male; priority journal; article; protein eso1; protein eso2 |
| Journal Title: | Gene |
| Volume: | 297 |
| Issue: | 1-2 |
| ISSN: | 0378-1119 |
| Publisher: | Elsevier Science, Inc. |
| Date Published: | 2002-09-04 |
| Start Page: | 141 |
| End Page: | 149 |
| Language: | English |
| DOI: | 10.1016/s0378-1119(02)00879-x |
| PUBMED: | 12384295 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 14 November 2014 -- Source: Scopus |
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