Identification of multiple cancer/testis antigens by allogeneic antibody screening of a melanoma cell line library Journal Article
| Authors: | Chen, Y. T.; Güre, A. O.; Tsang, S.; Stockert, E.; Jäger, E.; Knuth, A.; Old, L. J. |
| Article Title: | Identification of multiple cancer/testis antigens by allogeneic antibody screening of a melanoma cell line library |
| Abstract: | Cancer/testis (CT) antigens - immunogenic protein antigens that are expressed in testis and a proportion of diverse human cancer types - are promising targets for cancer vaccines. To identify new CT antigens, we constructed an expression cDNA library from a melanoma cell line that expresses a wide range of CT antigens and screened the library with an allogeneic melanoma patient serum known to contain antibodies against two CT antigens, MAGE-1 and NY-ESO-1. cDNA clones isolated from this library identified four CT antigen genes: MAGE-4a, NY-ESO-1, LAGE-1, and CT7. Of these four, only MAGE-4a and NY-ESO-1 proteins had been shown to be immunogenic. LAGE-1 is a member of the NY-ESO-1 gene family, and CT7 is a newly defined gene with partial sequence homology to the MAGE family at its carboxyl terminus. The predicted CT7 protein, however, contains a distinct repetitive sequence at the 5' end and is much larger than MAGE proteins. Our findings document the immunogenicity of LAGE-1 and CT7 and emphasize the power of serological analysis of cDNA expression libraries in identifying new human tumor antigens. |
| Keywords: | human cell; sequence analysis; antigen expression; melanoma; reverse transcription polymerase chain reaction; carboxy terminal sequence; cancer screening; tumor cells, cultured; gene library; tumor antigen; amino acid sequence; molecular sequence data; antigen; antigens; antigens, neoplasm; immunogenicity; serodiagnosis; antibodies; sequence homology; alloantigen; testis; dna, complementary; dna library; allogeneic restriction; recombinant dna; humans; human; male; priority journal; article; serological analysis of recombinant cdna expression libraries; human cancer immunology |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 95 |
| Issue: | 12 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1998-06-09 |
| Start Page: | 6919 |
| End Page: | 6923 |
| Language: | English |
| DOI: | 10.1073/pnas.95.12.6919 |
| PUBMED: | 9618514 |
| PROVIDER: | scopus |
| PMCID: | PMC22686 |
| DOI/URL: | |
| Notes: | Article -- Export Date: 12 December 2016 -- Source: Scopus |
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