Cancer/testis antigen expression and autologous humoral immunity to NY-ESO-1 in gastric cancer Journal Article
| Authors: | Wang, Y.; Wu, X. J.; Zhao, A. L.; Yuan, Y. H.; Chen, Y. T.; Jungbluth, A. A.; Gnjatic, S.; Santiago, D.; Ritter, G.; Chen, W. F.; Old, L. J.; Ji, J. F. |
| Article Title: | Cancer/testis antigen expression and autologous humoral immunity to NY-ESO-1 in gastric cancer |
| Abstract: | Gastric cancer has the highest mortality rate and the second-highest morbidity rate of all malignant tumors in China. Since cancer/testis (CT) antigens are expressed in various types of human tumors but generally not in normal tissue except for testis, they are promising antigens for cancer immunotherapy. NY-ESO-1, in particular, is the most immunogenic of the CT antigens. To study the feasibility of developing a CT antigen vaccine for gastric cancer, 101 gastric cancer samples were analyzed for the presence of NY-ESO-1 mRNA and that of 10 other CT antigen genes. Twelve out of 101 samples (11.9%) were found to be NY-ESO-1 mRNA-positive, 11 of them from advanced stage patients. In 7 of the 12 NY-ESO-1 mRNA-positive samples, the NY-ESO-1 protein was also detected by immunohistochemistry. An autologous humoral immune response to NY-ESO-1 was detected in 6 of 12 advanced stage NY-ESO-1 mRNA-positive patients, indicating that NY-ESO-1 is immunogenic in advanced stage gastric cancer. The serum from a patient with an NY-ESO-1 negative but LAGE-1 positive tumor was also found to be NY-ESO-1 antibody positive, possibly due to cross-reactivity between NY-ESO-1 and LAGE-1. All NY-ESO-1 mRNA-positive gastric cancer samples also expressed one to seven additional CT genes, revealing a tendency toward a clustered expression pattern, regardless of disease stage. About 74% of the samples expressed at least one CT antigen, most frequently MAGE-3 (41.6%). NY-ESO-1 and MAGE-3 are thus potential targets for a multivalent CT antigen vaccine. |
| Keywords: | immunohistochemistry; controlled study; unclassified drug; gene mutation; cancer staging; antigen expression; gene expression; tumor antigen; cancer vaccine; tumor antigens; cancer testis antigen; ny eso 1 antigen; stomach cancer; cross reaction; humoral immunity; gastric cancer; cancer/testis; immunogenetics; lage 1 antigen; human; article |
| Journal Title: | Cancer Immunity |
| Volume: | 4 |
| ISSN: | 1424-9634 |
| Publisher: | Academy of Cancer Immunology |
| Date Published: | 2004-11-01 |
| Start Page: | 11 |
| End Page: | 17 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 15516106 |
| DOI/URL: | |
| Notes: | Cancer Immun. -- Cited By (since 1996):6 -- Export Date: 16 June 2014 -- Source: Scopus |
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