NY-ESO-1 and LAGE-1 cancer-testis antigens are potential targets for immunotherapy in epithelial ovarian cancer Journal Article

Authors: Odunsi, K.; Jungbluth, A. A.; Stockert, E.; Qian, F.; Gnjatic, S.; Tammela, J.; Intengan, M.; Beck, A.; Keitz, B.; Santiago, D.; Williamson, B.; Scanlan, M. J.; Ritter, G.; Chen, Y. T.; Driscoll, D.; Sood, A.; Lele, S.; Old, L. J.
Article Title: NY-ESO-1 and LAGE-1 cancer-testis antigens are potential targets for immunotherapy in epithelial ovarian cancer
Abstract: Cancer-testis (CT) antigens are expressed in a variety of cancers, but not in normal adult tissues, except for germ cells of the testis, and hence appear to be ideal targets for immunotherapy. In an effort to examine the potential of NY-ESO-1 and LAGE-1 CT antigens for immunotherapy in epithelial ovarian cancer (EOC), we examined the expression of these antigens by reverse transcription-PCR (RT-PCR) and immunohistochemistry (IHC) in a large panel of EOC tissues and cell lines. Sera from a subgroup of the patients were tested for NY-ESO-1/LAGE-1 antibody by ELISA. The data indicated that four ovarian cancer cell lines were positive for one or both CT antigens. Expression of NY-ESO-1 in EOC was demonstrated by RT-PCR and/or IHC in 82 of 190 (43%) specimens. NY-ESO-1 expression by IHC ranged from homogeneous to heterogeneous pattern. LAGE-1 mRNA expression was present in 22 of 107 (21%) tumor tissues. Overall, the expression of either NY-ESO-1 or LAGE-1 mRNA was present in 42 of 107 (40%) EOC specimens and coexpression of both antigens was demonstrated in 11% of specimens. Antibody to NY-ESO-1/LAGE-1 was present in 11 of 37 (30%) patients whose tumors expressed either NY-ESO-1 or LAGE-1. Detectable antibodies were present for up to 3 years after initial diagnosis. Although there was no statistically significant relation between expression of NY-ESO-1/LAGE-1 antigen and survival, the data showed aberrant expression of NY-ESO-1 and LAGE-1 by IHC/RT-PCR in a significant proportion of EOC patients. These findings indicate that NY-ESO-1 and LAGE-1 are attractive targets for antigen-specific immunotherapy in EOC.
Keywords: adult; cancer survival; controlled study; human tissue; aged; aged, 80 and over; middle aged; unclassified drug; human cell; major clinical study; drug targeting; ovarian neoplasms; proteins; cancer immunotherapy; reverse transcription polymerase chain reaction; gene expression; membrane proteins; tumor antigen; enzyme linked immunosorbent assay; gene expression regulation; correlation analysis; antigens, neoplasm; messenger rna; diagnostic value; rna, messenger; cancer testis antigen; ny eso 1 antigen; antigen detection; tumor cell line; ovary carcinoma; protein biosynthesis; enzyme-linked immunosorbent assay; antibodies, neoplasm; antigens, surface; antigen function; lage 1 antigen; humans; prognosis; human; female; priority journal; article
Journal Title: Cancer Research
Volume: 63
Issue: 18
ISSN: 0008-5472
Publisher: American Association for Cancer Research  
Date Published: 2003-09-15
Start Page: 6076
End Page: 6083
Language: English
PUBMED: 14522938
PROVIDER: scopus
Notes: Export Date: 12 September 2014 -- Source: Scopus