Immunohistochemical analysis of NY-ESO-1 antigen expression in normal and malignant human tissues Journal Article
| Authors: | Jungbluth, A. A.; Chen, Y. T.; Stockert, E.; Busam, K. J.; Kolb, D.; Iversen, K.; Coplan, K.; Williamson, B.; Altorki, N.; Old, L. J. |
| Article Title: | Immunohistochemical analysis of NY-ESO-1 antigen expression in normal and malignant human tissues |
| Abstract: | NY-ESO-I, a member of the CT (cancer/testis) family of antigens, is expressed in normal testis and in a range of human tumor types. Knowledge of NY-ESO- I expression has depended on RT-PCR detection of mRNA and there is a need for detecting NY-ESO-I at the protein level. In the present study, a method for the immunochemical detection of NY-ESO-I in paraffin-embedded tissues has been developed and used to define the expression pattern of NY-ESO- I in normal tissues and in a panel of human tumors. No normal tissue other than testis showed NY-ESO-I reactivity, and expression in testis was restricted to germ cells particularly spermatogonia. In human tumors, the frequency of NY-ESO-I antigen expression corresponds with past analysis of NY-ESO-I mRNA expression e.g., 20-30% of lung cancers, bladder cancers and melanoma, and no expression in colon and renal cancer. Co-typing of NY-ESO-I antigen and mRNA expression in a large panel of lung cancers showed a good correlation. There is great variability in NY-ESO-I expression in individual tumors, ranging from an infrequent homogeneous pattern of staining to highly heterogeneous antigen expression. © 2001 Wiley-Liss, Inc. |
| Keywords: | immunohistochemistry; controlled study; protein expression; unclassified drug; human cell; antigen expression; melanoma; reverse transcription polymerase chain reaction; germ cell; lung neoplasms; colonic neoplasms; membrane proteins; lung cancer; tumor cells, cultured; immunoreactivity; breast neoplasms; bladder cancer; urinary bladder neoplasms; kidney neoplasms; antibodies, monoclonal; antigen; antigens, neoplasm; tissue distribution; carcinoma, renal cell; messenger rna; reverse transcriptase polymerase chain reaction; head and neck neoplasms; colon cancer; ny eso 1 antigen; antigen detection; protein biosynthesis; kidney cancer; protein determination; testis; ny-eso-1; tumor-associated antigen; spermatogonia; ct-antigen; humans; human; male; female; priority journal; article; es121 |
| Journal Title: | International Journal of Cancer |
| Volume: | 92 |
| Issue: | 6 |
| ISSN: | 0020-7136 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2001-06-15 |
| Start Page: | 856 |
| End Page: | 860 |
| Language: | English |
| DOI: | 10.1002/ijc.1282 |
| PUBMED: | 11351307 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 21 May 2015 -- Source: Scopus |
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