A testicular antigen aberrantly expressed in human cancers detected by autologous antibody screening Journal Article
| Authors: | Chen, Y. T.; Scanlan, M. J.; Sahin, U.; Türeci, Ö; Gure, A. O.; Tsang, S.; Williamson, B.; Stockert, E.; Pfreundschuh, M.; Old, L. J. |
| Article Title: | A testicular antigen aberrantly expressed in human cancers detected by autologous antibody screening |
| Abstract: | Serological analysis of recombinant cDNA expression libraries (SEREX) using tumor mRNA and autologous patient serum provides a powerful approach to identify immunogenic tumor antigens. We have applied this methodology to a case of esophageal squamous cell carcinoma and identified several candidate tumor targets. One of these, NY-ESO-1, showed restricted mRNA expression in normal tissues, with high-level mRNA expression found only in testis and ovary tissues. Reverse transcription-PCR analysis showed NY-ESO-1 mRNA expression in a variable proportion of a wide array of human cancers, including melanoma, breast cancer, bladder cancer, prostate cancer, and hepatocellular carcinoma. NY-ESO-1 encodes a putative protein of Mr 17,995 having no homology with any known protein. The pattern of NY-ESO-1 expression indicates that it belongs to an expanding family of immunogenic testicular antigens that are aberrantly expressed in human cancers in a lineage- nonspecific fashion. These antigens, initially detected by either cytotoxic T cells (MAGE, BAGE, GAGE-1) or antibodies [HOM-MEL-40(SSX2), NY-ESO-1], represent a pool of antigenic targets for cancer vaccination. |
| Keywords: | adult; human tissue; sequence analysis; case report; carcinoma, squamous cell; conference paper; polymerase chain reaction; neoplasms; proteins; reverse transcription polymerase chain reaction; gene expression; membrane proteins; gene product; gene library; tumor antigen; ovary; cloning, molecular; gene expression regulation, neoplastic; amino acid sequence; molecular sequence data; antigens, neoplasm; tissue distribution; messenger rna; rna, messenger; antigen detection; base sequence; blotting, northern; molecular weight; esophagus carcinoma; serology; antibody; testis; esophageal neoplasms; normal human; complementary dna; dna, complementary; testis antigens; cancer; humans; human; male; female; priority journal |
| Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
| Volume: | 94 |
| Issue: | 5 |
| ISSN: | 0027-8424 |
| Publisher: | National Academy of Sciences |
| Date Published: | 1997-03-04 |
| Start Page: | 1914 |
| End Page: | 1918 |
| Language: | English |
| DOI: | 10.1073/pnas.94.5.1914 |
| PUBMED: | 9050879 |
| PROVIDER: | scopus |
| PMCID: | PMC20017 |
| DOI/URL: | |
| Notes: | Conference Paper -- Export Date: 17 March 2017 -- Source: Scopus |
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