NY-ESO-1 expression and immunogenicity in esophageal cancer Journal Article
| Authors: | Fujita, S.; Wada, H.; Jungbluth, A. A.; Sato, S.; Nakata, T.; Noguchi, Y.; Doki, Y.; Yasui, M.; Sugita, Y.; Yasuda, T.; Yano, M.; Ono, T.; Chen, Y. T.; Higashiyama, M.; Gnjatic, S.; Old, L. J.; Nakayama, E.; Monden, M. |
| Article Title: | NY-ESO-1 expression and immunogenicity in esophageal cancer |
| Abstract: | Purpose: Although NY-ESO-1 was isolated from an esophageal carcinoma patient, its expression in this type of cancer and its immunogenicity in esophageal cancer patients have not yet been fully elucidated. We report here the frequency of NY-ESO-1 mRNA and protein expression in esophageal cancer and the presence of NY-ESO-1-specific immune response in patients. Experimental Design: One hundred twenty three esophageal squamous cell carcinoma specimens were analyzed for the expression of NY-ESO-1 mRNA by conventional and real-time reverse transcription-PCR and the expression of protein by immunohistochemistry and Western blot. Sera and peripheral blood lymphocytes from 51 patients were analyzed for the NY-ESO-1 antibody production by enzyme-linked immunosorbent assay and NY-ESO-1 T cell response by enzyme-linked immunospot assay. Survival analyses were also performed. Results: NY-ESO-1 mRNA was expressed in 41 of 123 (33%) esophageal squamous cell carcinoma specimens, and its expression was found at higher frequency in well-differentiated and moderately differentiated type of cancer. No mRNA copy was detected in any of the adjacent normal tissues. Twenty-one of 24 (87.5%) NY-ESO-1 mRNA-positive tumors were stained positively by immunohistochemistry. Correlation between the level of NY-ESO-1 mRNA expression and the degree of immunohistochemistry positivity was observed. Antibody production was observed in 2 patients with famers that showed protein expression. Furthermore, a CD8 T-cell response against NY-ESO-1 was observed in 1 of the 2 seropositive patients. Conclusions: The high expression frequency of NY-ESO-1 mRNA and protein indicates NY-ESO-1 as a feasible vaccine target in esophageal cancer. |
| Keywords: | immunohistochemistry; cancer survival; clinical article; controlled study; human tissue; protein expression; survival analysis; unclassified drug; human cell; squamous cell carcinoma; follow-up studies; polymerase chain reaction; t lymphocyte; cd8-positive t-lymphocytes; reverse transcription polymerase chain reaction; membrane proteins; gene frequency; tumor antigen; enzyme linked immunosorbent assay; blotting, western; gene expression regulation, neoplastic; correlation analysis; immune response; antigens, neoplasm; antigen specificity; messenger rna; reverse transcriptase polymerase chain reaction; rna, messenger; ny eso 1 antigen; antigen detection; blood analysis; immunogenicity; western blotting; antibodies; enzyme-linked immunosorbent assay; enzyme linked immunospot assay; lymphocyte; esophagus carcinoma; esophageal neoplasms; antibody production; seroprevalence; humans; human; priority journal; article |
| Journal Title: | Clinical Cancer Research |
| Volume: | 10 |
| Issue: | 19 |
| ISSN: | 1078-0432 |
| Publisher: | American Association for Cancer Research |
| Date Published: | 2004-10-01 |
| Start Page: | 6551 |
| End Page: | 6558 |
| Language: | English |
| DOI: | 10.1158/1078-0432.ccr-04-0819 |
| PROVIDER: | scopus |
| PUBMED: | 15475443 |
| DOI/URL: | |
| Notes: | Clin. Cancer Res. -- Cited By (since 1996):34 -- Export Date: 16 June 2014 -- CODEN: CCREF -- Source: Scopus |
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