Expression and immunogenicity of NY-ESO-1 in hepatocellular carcinoma Journal Article
| Authors: | Nakamura, S.; Nouso, K.; Noguchi, Y.; Higashi, T.; Ono, T.; Jungbluth, A.; Chen, Y. T.; Old, L. J.; Nakayama, E.; Shiratori, Y. |
| Article Title: | Expression and immunogenicity of NY-ESO-1 in hepatocellular carcinoma |
| Abstract: | Background and Aim: The present study was designed to investigate the expression of and humoral response against NY-ESO-1 in patients with hepatocellular carcinoma and to analyze the relationship between expression of NY-ESO-1 mRNA and clinicopathological features. Methods: NY-ESO-1 mRNA and protein expression in surgically resected hepatocellular carcinoma specimens, adjacent non-cancerous liver and non-tumor bearing liver were examined by reverse transcription-polymerase chain reaction and immunohistochemical staining using a monoclonal antibody against NY-ESO-1 (ES121), respectively. The antibody response to NY-ESO-1 was examined by enzyme-linked immunosorbent assay using recombinant NY-ESO-1 protein. Results: NY-ESO-1 mRNA was detected in 18 of 41 (43.9%) hepatocellular carcinomas. No NY-ESO-1 mRNA was expressed in 41 paired non-cancerous specimens and 18 specimens histologically diagnosed as liver cirrhosis or chronic hepatitis. Immunohistochemistry revealed heterogeneous expression of NY-ESO-1 protein in three of 18 NY-ESO-1 mRNA-positive hepatocellular carcinomas. None of 23 NY-ESO-1 mRNA-negative hepatocellular carcinomas expressed NY-ESO-1 protein. Antibody against NY-ESO-1 protein was detected in two of 92 patients with hepatocellular carcinoma. Both of these patients had tumors invading main branches of the portal vein. Conclusions: The present study has demonstrated the expression of NY-ESO-1 mRNA in hepatocellular carcinoma and NY-ESO-1 antibody production in patients with advanced hepatocellular carcinoma. Although the enhancement of NY-ESO-1 protein expression and the activation of immune response of the patients with hepatocellular carcinoma are necessary, NY-ESO-1 has the potential to be a good target molecule for immunotherapy against advanced hepatocellular carcinoma. © 2006 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd. |
| Keywords: | immunohistochemistry; adult; clinical article; controlled study; human tissue; protein expression; aged; middle aged; clinical feature; histopathology; liver cell carcinoma; liver cirrhosis; carcinoma, hepatocellular; liver neoplasms; neoplasm; antigen expression; protein analysis; reverse transcription polymerase chain reaction; gene expression; membrane proteins; enzyme linked immunosorbent assay; cancer invasion; tissue section; liver; immunotherapy; antigens, neoplasm; messenger rna; reverse transcriptase polymerase chain reaction; ny eso 1 antigen; immunogenicity; antibody response; recombinant protein; dna, neoplasm; antibody detection; humoral immunity; portal vein; chronic hepatitis; cancer/ testis antigens |
| Journal Title: | Journal of Gastroenterology and Hepatology |
| Volume: | 21 |
| Issue: | 8 |
| ISSN: | 0815-9319 |
| Publisher: | Wiley Blackwell |
| Date Published: | 2006-08-01 |
| Start Page: | 1281 |
| End Page: | 1285 |
| Language: | English |
| DOI: | 10.1111/j.1440-1746.2006.04271.x |
| PUBMED: | 16872310 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 7" - "Export Date: 4 June 2012" - "CODEN: JGHEE" - "Source: Scopus" |
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