Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number Journal Article
| Authors: | Sato, S.; Noguchi, Y.; Wada, H.; Fujita, S.; Nakamura, S.; Tanaka, R.; Nakada, T.; Hasegawa, K.; Nakagawa, K.; Koizumi, F.; Ono, T.; Nouso, K.; Jungbluth, A.; Chen, Y. T.; Old, L. J.; Shiratori, Y.; Nakayama, E. |
| Article Title: | Quantitative real-time RT-PCR analysis of NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and tumors, and correlation of the protein expression with the mRNA copy number |
| Abstract: | We investigated NY-ESO-1 and LAGE-1a mRNA expression in normal tissues and various types of cancer by quantitative real-time RT-PCR. In addition to their high expression in the testis, we observed a low expression of NY-ESO-1 mRNA in the placenta, pancreas and liver, and no expression in 12 other normal tissues. We also observed a low expression of LAGS-1a mRNA in the placenta and ovary, and marginal expression in 13 other normal tissues. In contrast to the previous finding that NY-ESO-1 and LAGS-1a mRNAs were mostly co-expressed in solid tumors, we found an independent expression of NY-ESO-1 and LAGS-1a mRNAs. NY-ESO-1 mRNA expression was mostly associated with LAGS-1a mRNA expression in esophageal and liver cancers, but not in prostate cancer. Immunohistochemistry (IHC) using NY-ESO-1-specific ES121 mAb showed that NY-ESO-1 protein was detected in 6 of 9 and 3 of 10 NY-ESO-1 mRNA-positive specimens from esophageal and liver cancers, respectively. NY-ESO-1 protein expression was correlated with the copy numbers of NY-ESO-1 mRNA. IHC was also performed using ES121 mAb and B9.8 mAb recognizing both NY-ESO-1 and LAGE-1a in 4 esophageal and 6 liver cancer specimens preferentially expressing LAGE-1a mRNA. B9.8-specific staining was observed weakly and focally in one liver cancer specimen expressing >10(5) copies of LAGS-1a mRNA. |
| Keywords: | immunohistochemistry; immunotherapy; cancer/testis antigens; epitopes; identification; gene-expression; cell carcinoma; metastatic melanoma; monoclonal-antibodies; ny-eso-1; cancer-testis antigens; immune-response; glyceraldehyde-3-phosphate dehydrogenase; lage-1a; real-time rt-pcr |
| Journal Title: | International Journal of Oncology |
| Volume: | 26 |
| Issue: | 1 |
| ISSN: | 1019-6439 |
| Publisher: | Spandidos Publications |
| Date Published: | 2005-01-01 |
| Start Page: | 57 |
| End Page: | 63 |
| Language: | English |
| ACCESSION: | WOS:000226104600007 |
| PROVIDER: | wos |
| PUBMED: | 15586225 |
| Notes: | --- - Article - "Source: Wos" |
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