Physical interaction of two cancer-testis antigens, MAGE-C1 (CT7) and NY-ESO-1 (CT6) Journal Article

Authors: Cho, H. J.; Caballero, O. L.; Gnjatic, S.; Andrade, V. C. C.; Colleoni, G. W.; Vettore, A. L.; Outtz, H. H.; Fortunato, S.; Altorki, N.; Ferrera, C. A.; Chua, R.; Jungbluth, A. A.; Chen, Y. T.; Old, L. J.; Simpson, A. J. G.
Article Title: Physical interaction of two cancer-testis antigens, MAGE-C1 (CT7) and NY-ESO-1 (CT6)
Abstract: Cancer/testis (CT) antigens are the protein products of germ line-associated genes that are activated in a wide variety of tumors and can elicit autologous cellular and humoral immune responses. CT antigens can be divided between those that are encoded on the X chromosome (CT-X antigens) and those that are not (non-X CT antigens). Among the CT-X antigens, the melanoma antigen gene (MAGE) family, defined by a shared MAGE homology domain (MHD), is the largest. CT-X genes are frequently expressed in a coordinate manner in cancer cells, and their expression appears to be modulated by epigenetic mechanisms. The expression of CT-X genes is associated with advanced disease and poor outcome in different tumor types. We used the yeast two-hybrid system to identify putative MHD-interacting proteins. The MHD of MAGE-C1 (CT7) was used as bait to screen a human testis cDNA library. This study identified NY-ESO-1 (CT6) as a MAGE-C1 binding partner. Immunoprecipitation and immunofluorescence staining confirmed MAGE-C1 interaction with NY-ESO-1, and cytoplasmic co-localization of both proteins in melanoma cells. Co-expression of these two genes was found to occur in cancer cell lines from different origins, as well as in primary tumors (multiple myeloma and non-small cell lung cancer samples). This is the first report of direct interaction between two CT antigens and may be pertinent in the light of the frequently coordinated expression of these proteins. © 2006 by Andrew J. G. Simpson.
Keywords: controlled study; human tissue; human cell; major clinical study; protein domain; protein localization; multiple myeloma; gene expression; protein protein interaction; lung non small cell cancer; genetic association; cancer cell culture; immunofluorescence; gene activation; cellular immunity; epigenetics; cancer testis antigen; ny eso 1 antigen; nucleotide sequence; immunoprecipitation; x chromosome; melanoma antigen; sequence homology; antigen binding; humoral immunity; genetic screening; cell lysate; rt-pcr; mage c1 antigen; ny-eso-1; nsclc; mage-c1; dna library; two-hybrid assay
Journal Title: Cancer Immunity
Volume: 6
ISSN: 1424-9634
Publisher: Academy of Cancer Immunology  
Date Published: 2006-12-01
Language: English
PROVIDER: scopus
PUBMED: 17137291
Notes: --- - "Cited By (since 1996): 6" - "Export Date: 4 June 2012" - "Source: Scopus"