Changes in mouse mu opioid receptor exon 7/8-like immunoreactivity following food restriction and food deprivation in rats Journal Article
| Authors: | Hadjimarkou, M. M.; Abbadie, C.; Kasselman, L. J.; Pan, Y. X.; Pasternak, G. W.; Bodnar, R. J. |
| Article Title: | Changes in mouse mu opioid receptor exon 7/8-like immunoreactivity following food restriction and food deprivation in rats |
| Abstract: | Opioid agonists and antagonists respectively increase and decrease food intake. That selective mu opioid antagonists are more effective than antisense probes directed against the mu opioid receptor (MOR-1) gene in reducing deprivation-induced feeding suggests a role for isoforms. Both food restriction and deprivation alter protein and mRNA levels of opioid peptides and receptors. Antisera directed against Exon 4 of the MOR-1-like immunoreactivity (LI) (Exon 4) clone or directed against mouse Exons 7/8 (mE7/8-LI) revealed high levels of immunoreactivity in brain nuclei related to feeding behavior. Therefore, the present study assessed MOR-1LI and mE7/8-LI in hypothalamic and extrahypothalamic sites in rats exposed to ad libitum feeding, food restriction (2, 7, 14 days), or food deprivation (24, 48 h). MOR-1-LI displayed robust reactivity, but was insensitive to food restriction or deprivation. mE7/8-LI, both in terms of cell counts and relative optical density, was significantly and selectively increased in the dorsal and ventral parvocellular subdivisions of the hypothalamic paraventricular nucleus in food-restricted (14 days) rats, but all other restriction or deprivation regimens were ineffective in other hypothalamic nuclei. In contrast, significant and site-specific decreases in relative optical density in the rostral part of the nucleus tractus solitarius (NTS) were observed in food-restricted (2, 7 days) or food-deprived (24, 48 h) animals, but these regimens were ineffective in the other extrahypothalamic sites. This study indicates the sensitivity of this mE7/8-LI probe in the hypothalamic parvocellular paraventricular nucleus and rostral NTS to food restriction and deprivation in rats. ©2009 Wiley-Liss, Inc. |
| Keywords: | immunohistochemistry; controlled study; unclassified drug; exon; exons; nonhuman; animal cell; animals; mice; animal tissue; animal experiment; animal model; body weight; immunoreactivity; feeding; food intake; drug receptor binding; gene expression regulation; molecular cloning; diet; brain; messenger rna; hypothalamus; rat; cell count; rats; rats, sprague-dawley; opioids; nucleus tractus solitarius; paraventricular hypothalamic nucleus; antiserum; mu opiate receptor; mu opiate receptor 1 like immunoreactivity antiserum; mu opiate receptor 1c; mu opiate receptor agonist; mu opiate receptor antagonist; mu opiate receptor exon 7/8 like immunoreactivity antiserum; anterior hypothalamus; brain nucleus; brain region; diet restriction; food deprivation; molecular probe; mor 1 gene; optical density; receptor density; receptor gene; solitary tract nucleus; fasting; photomicrography; receptors, opioid, mu; solitary nucleus |
| Journal Title: | Synapse |
| Volume: | 63 |
| Issue: | 7 |
| ISSN: | 0887-4476 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2009-07-01 |
| Start Page: | 585 |
| End Page: | 597 |
| Language: | English |
| DOI: | 10.1002/syn.20639 |
| PUBMED: | 19301417 |
| PROVIDER: | scopus |
| PMCID: | PMC2749570 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 30 November 2010" - "CODEN: SYNAE" - "Source: Scopus" |
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