Differential distribution in rat brain of mu opioid receptor carboxy terminal splice variants MOR-1C-like and MOR-1-like immunoreactivity: Evidence for region-specific processing Journal Article
| Authors: | Abbadie, C.; Pan, Y. X.; Pasternak, G. W. |
| Article Title: | Differential distribution in rat brain of mu opioid receptor carboxy terminal splice variants MOR-1C-like and MOR-1-like immunoreactivity: Evidence for region-specific processing |
| Abstract: | The present study examined immunohistochemically the regional distribution of the mu opioid receptor splice variant MOR-1C by using a rabbit antisera generated against the C-terminal peptide sequences and compared it with MOR-1. Overall, the distribution of MOR-1C-like immunoreactivity (-LI) differed from MOR-1-LI. Both MOR-1C-LI and MOR-1-LI were prominent in a few central nervous system regions, including the lateral parabrachial nucleus, the periaqueductal gray, and laminae I-II of the spinal trigeminal nuclei and the spinal cord. In the striatum, hippocampal formation, presubiculum and parasubiculum, amygdaloid nuclei, thalamic nuclei, locus coeruleus, and nucleus ambiguous MOR-1-LI predominated, whereas MOR-1C-LI was absent or sparse. Conversely, MOR-1C-LI exceeded MOR-1-LI in the lateral septum, the deep laminae of the spinal cord, and most hypothalamic nuclei such as the median eminence, periventricular, suprachiasmatic, supraoptic, arcuate, paraventricular, ventromedial, and dorsomedial nuclei. Double-labeling studies showed colocalization of the two receptors in neurons of the lateral septum, but not in the median eminence or in the arcuate nucleus, even though both MOR-1 isoforms were expressed. Because both MOR-1 and MOR-1C are derived from the same gene, these differences in regional distribution represent region-specific mRNA processing. The regional distributions reported in this study involve the epitope seen by the combinations of exons 7, 8, and 9. However, if other MOR- 1 variants containing exons 7, 8, and 9 exist, the antisera would not distinguish between them and MOR-1C. (C) 2000 Wiley-Liss, Inc. |
| Keywords: | immunohistochemistry; exon; nonhuman; protein localization; animal cell; mouse; animals; animal tissue; carboxy terminal sequence; central nervous system; protein processing; amino acid sequence; molecular sequence data; protein processing, post-translational; tissue distribution; brain; peptide fragments; rat; alternative splicing; immunoblotting; rats; mu opiate receptor; receptors, opioid, mu; cho cells; cricetinae; rabbits; variation (genetics); chemical labeling; splice variant; dna, recombinant; mu-opioid receptor; male; priority journal; article; double labeling |
| Journal Title: | Journal of Comparative Neurology |
| Volume: | 419 |
| Issue: | 2 |
| ISSN: | 0021-9967 |
| Publisher: | John Wiley & Sons |
| Date Published: | 2000-04-03 |
| Start Page: | 244 |
| End Page: | 256 |
| Language: | English |
| DOI: | 10.1002/(sici)1096-9861(20000403)419:2<244::aid-cne8>3.0.co;2-r |
| PUBMED: | 10723002 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Export Date: 18 November 2015 -- Source: Scopus |
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