Immunohistochemical labeling of the mu opioid receptor carboxy terminal splice variant mMOR-1B4 in the mouse central nervous system Journal Article

  


Authors: Zhang, Y.; Pan, Y. X.; Kolesnikov, Y.; Pasternak, G. W.
Article Title: Immunohistochemical labeling of the mu opioid receptor carboxy terminal splice variant mMOR-1B4 in the mouse central nervous system
Abstract: The mu opioid receptor gene Oprm is alternatively spliced into many variants, providing for the multiplicity of mu opioid receptor subtypes. One of the mouse variants, mMOR-1B4, is unique in that it displays high affinity towards a wide range of mu opioid receptor antagonists, but poor affinity towards most classical mu opioid agonists. The present study examined the immunohistochemical distribution of the mMOR-1B4 variant in mouse brain and spinal cord. mMOR-1B4-like immunoreactivity (mMOR-1B4-LI) was enriched in many regions of the brain, spinal cord and in the dorsal root ganglia. Some of the structures showing prominent mMOR-1B4-LI include the olfactory bulb, cerebral cortex, bed nucleus of stria terminalis, hippocampus, habenular nucleus, amygdala, thalamus, hypothalamus, medium eminence, substantia nigra, ventral tegmental area, oculomotor nucleus, red nucleus, raphe nuclei, periaqueductal gray, locus coeruleus, trigeminal nucleus, reticular formation, area postrema and Purkinje cell layer and deep nuclei of cerebellum. mMOR-1B4-LI was present in afferent neurons of the dorsal root ganglia and their projecting fibers into the superficial laminae of the spinal dorsal horn. Some motor neurons in the anterior horn of the spinal cord also were immunopositive. The overall distribution of mMOR-1B4-LI in the central nervous system is distinguishable from previously characterized variants such as MOR-1-LI, MOR-1C-LI and exon-11-LI. These studies provide evidence for the region- and neuron-specific processing of the Oprm gene and support the possibility of functional differences among the variants. © 2006 Elsevier B.V. All rights reserved.
Keywords: immunohistochemistry; controlled study; unclassified drug; nonhuman; protein localization; mouse; animals; mice; animal tissue; cerebellum; purkinje cell; carboxy terminal sequence; opiate; brain cortex; central nervous system; tissue distribution; brain; sensory nerve; spinal cord; peptide fragments; hypothalamus; locus ceruleus; alternative splicing; morphine; protein variant; mu opiate receptor; brain nucleus; receptors, opioid, mu; rna splicing; olfactory bulb; motoneuron; nerve projection; opioid receptor; hippocampus; clone; spinal ganglion; thalamus; spinal cord dorsal horn; mor-1; variation (genetics); amygdaloid nucleus; periaqueductal gray matter; substantia nigra; splice variant; mu opiate receptor 1b4; receptor subunit; anterior horn cell; area postrema; habenula; oculomotor nucleus; raphe nucleus; red nucleus; reticular formation; stria terminalis; trigeminal nucleus; ventral tegmentum
Journal Title: Brain Research
Volume: 1099
Issue: 1
ISSN: 0006-8993
Publisher: Elsevier Science, Inc.  
Date Published: 2006-07-12
Start Page: 33
End Page: 43
Language: English
DOI: 10.1016/j.brainres.2006.04.133
PUBMED: 16793025
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-33746093526&partnerID=40&md5=1df7c97a952798f756c5ee79902141d7
Notes: --- - "Cited By (since 1996): 23" - "Export Date: 4 June 2012" - "CODEN: BRREA" - "Source: Scopus"
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MSK Authors
  1. Yahong Zhang
    6 Zhang
  2. Yuri Alexei Kolesnikov
    34 Kolesnikov
  3. Yingxian Pan
    132 Pan
  4. Gavril W Pasternak
    414 Pasternak
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