Anatomical and functional correlation of the endomorphins with mu opioid receptor splice variants Journal Article

  


Authors: Abbadie, C.; Rossi, G. C.; Orciuolo, A.; Zadina, J. E.; Pasternak, G. W.
Article Title: Anatomical and functional correlation of the endomorphins with mu opioid receptor splice variants
Abstract: The present study characterizes the relationship between the endogenous mu opioid peptides endomorphin-1 (EM-1) and endomorphin-2 (EM-2) and several splice variants of the cloned mu opioid receptor (MOR-1) encoded by the mu opioid receptor gene (Oprm). Confocal laser microscopy revealed that fibers containing EM-2-like immunoreactivity (-LI) were distributed in close apposition to fibers showing MOR-1-LI (exon 4-LI) and to MOR-1C-LI (exons 7/8/9-LI) in the superficial laminae of the lumbar spinal cord. We also observed colocalization of EM-2-LI and MOR-1-LI in a few fibers of lamina II, and colocalization of EM-2-LI and MOR-1C-LI in laminae I-II, and V-VI. To assess the functional relevance of the MOR-1 variants in endomorphin analgesia, we examined the effects of antisense treatments that targeted individual exons within the Oprm1 gene on EM-1 and EM-2 analgesia in the tail test. This antisense mapping study implied mu opioid receptor mechanisms for the endomorphins are distinct from those of morphine or morphine-6β-glucuronide (M6G).
Keywords: immunohistochemistry; controlled study; exon; exons; nonhuman; protein localization; mouse; animals; mice; pain; animal experiment; immunoreactivity; mice, inbred strains; correlation analysis; amino acid sequence; spinal cord; nucleotide sequence; alternative splicing; base sequence; protein structure, tertiary; morphine; oligopeptides; analgesia; mu opiate receptor; receptors, opioid, mu; tail flick test; oprm; cloning; mor-1; antisense; posterior horn cells; laser microscopy; glucuronide; male; priority journal; article; endomorphin 1; endomorphin 2; mor-1c; mop1
Journal Title: European Journal of Neuroscience
Volume: 16
Issue: 6
ISSN: 0953-816X
Publisher: Blackwell Publishing  
Date Published: 2002-09-01
Start Page: 1075
End Page: 1082
Language: English
DOI: 10.1046/j.1460-9568.2002.02173.x
PUBMED: 12383236
PROVIDER: scopus
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-0036455157&partnerID=40&md5=e333e28eeab2c2162eb346280dc1617e
Notes: Export Date: 14 November 2014 -- Source: Scopus
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MSK Authors
  1. Catherine Abbadie
    15 Abbadie
  2. Grace Rossi
    61 Rossi
  3. Gavril W Pasternak
    414 Pasternak
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