Performance of line-scanning confocal microscopy in human skin: Investigation of potential for clinical translation Conference Paper

  

Authors:	Larson, B.; Peterson, G.; Abeytunge, S.; Rajadhyaksha, M.
Title:	Performance of line-scanning confocal microscopy in human skin: Investigation of potential for clinical translation
Conference Title:	Advanced Biomedical and Clinical Diagnostic Systems IX
Abstract:	Line-scanning, using 8-10 optical components, linear-array detectors and custom-FPGA electronics, may enable smaller, simpler and lower-cost confocal microscopes to accelerate translation to the clinic. The adaptability of commercially available low-cost array detectors for confocal microscopy is being investigated. Measurements of optical sectioning and lateral resolution showed good agreement with theory, and are comparable to that of point-scanning systems. LSFs through full thickness of human epidermis show a two-fold degradation in sectioning performance. Imaging of human epidermis in vivo demonstrates nuclear and cellular detail down to the basal layer with a bench top setup and also a compact clinical prototype. Blood flow in oral mucosa can be imaged using the clinical prototype. However, speckle and background noise degrade contrast and resolution of the image. © 2011 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE).
Keywords:	microscopy; linescanning; scanning; confocal microscopy; skin; in-vivo; blood flow; endoscopy; confocal microscopes; human skin; human epidermis; lateral resolution; optical sectioning; scanning systems; basal layer; line-scanning microscopy; oral mucosa; array detectors; background noise; clinical translation; linear-array; optical components
Journal Title	Proceedings of SPIE
Volume:	7890
Conference Dates:	2011 Jan 23-25
Conference Location:	San Francisco, CA
ISBN:	0277-786X
Publisher:	SPIE
Date Published:	2011-01-01
Start Page:	78900O
Language:	English
DOI:	10.1117/12.876171
PROVIDER:	scopus
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-79955428560&partnerID=40&md5=5a6e8fa105ef603cb9e8f952a1b0af8f
Notes:	--- - Progress in Biomedical Optics and Imaging - Proceedings of SPIE - Progr. Biomed. Opt. Imaging Proc. SPIE - "Conference code: 84655" - "Export Date: 23 June 2011" - "Article No.: 78900O" - "Sponsors: The Society of Photo-Optical Instrumentation Engineers (SPIE)" - 23 January 2011 through 25 January 2011 - "Source: Scopus"

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