Full-pupil line-scanning confocal microscope for imaging weakly scattering tissues: Comparison to divided-pupil Conference Paper

  


Authors: Gareau, D. S.; Abeytunge, S.; Rajadhyaksha, M.
Title: Full-pupil line-scanning confocal microscope for imaging weakly scattering tissues: Comparison to divided-pupil
Conference Title: Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII
Abstract: Confocal reflectance full-pupil and divided-pupil line-scanning microscopes provide optical sectioning and image nuclear detail in skin. Line-scanning with linear detectors is a simpler alternative to point-scanning for imaging weakly scattering epidermis and the oral epithelium. With illumination of 830 nm, a water immersion lens of numerical aperture 0.9 and slit width three times smaller than the diffraction-limited line width, the instrumental full width at half maximum (FWHM) optical sectioning (linespread function) for the full-pupil design is 1.4 +/- 0.07 μm, which degrades through fullthickness human epidermis to 2.8 +/- 0.78 μm. The lateral resolution is 0.7±0.10 μm, which degrades to 1.6±0.28 μm through human epidermis. The divided-pupil design demonstrates instrumental optical sectioning of 1.7 μm, which degrades to 7.6 μm through human epidermis. The lateral resolution is 1.0 μm, which degrades to 1.7 μm. Heavy scattering in the dermis decreases contrast. Images of skin in-vivo show nuclear detail as expected with the predicted and experimentally verified sectioning. However, pixel crosstalk and speckle artifact degrade image quality in strongly scattering and aberrating tissues. The sources of degradation (aberration and scattering) are evaluated for the two design to assess the feasibility of these techniques for in vivo imaging. © 2009 SPIE.
Keywords: linescanning; scanning; confocal microscopy; histology; skin; image quality; in-vivo; diffraction limited; human epidermis; in-vivo imaging; lateral resolution; line spread functions; linear detectors; numerical aperture; optical sectioning; pixel cross-talk; scattering tissue; slit width; water-immersion lens; biomolecules; scattering
Journal Title Proceedings of SPIE
Volume: 7182
Conference Dates: 2009 Jan 26-28
Conference Location: San Jose, CA
ISBN: 0277-786X
Publisher: SPIE  
Date Published: 2009-01-01
Start Page: 718207
Language: English
DOI: 10.1117/12.809450
PROVIDER: scopus
PUBMED: 19506154
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-65949105483&partnerID=40&md5=0460e8cd62290e2e2df20885eeea8f93
Notes: --- - Progress in Biomedical Optics and Imaging - Proceedings of SPIE - "Export Date: 30 November 2010" - "Art. No.: 718207" - "CODEN: PSISD" - "Source: Scopus"
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MSK Authors
  1. Daniel S Gareau
    23 Gareau
  2. Milind Rajadhyaksha
    222 Rajadhyaksha
  3. Sanjeewa Abeytunge
    32 Abeytunge
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