Comparison of divided and full pupil configurations for line-scanning confocal microscopy in human skin and oral mucosa Conference Paper
| Authors: | Larson, B.; Abeytunge, S.; Glazowski, C.; Rajadhyaksha, M. |
| Title: | Comparison of divided and full pupil configurations for line-scanning confocal microscopy in human skin and oral mucosa |
| Conference Title: | Photonic Therapeutics and Diagnostics VIII |
| Abstract: | Confocal point-scanning microscopy has been showing promise in the detection, diagnosing and mapping of skin lesions in clinical settings. The noninvasive technique allows provides optical sectioning and cellular resolution for in vivo diagnosis of melanoma and basal cell carcinoma and pre-operative and intra-operative mapping of margins. The imaging has also enabled more accurate "guided" biopsies while minimizing the otherwise large number of "blind" biopsies. Despite these translational advances, however, point-scanning technology remains relatively complex and expensive. Line-scanning technology may offer an alternative approach to accelerate translation to the clinic. Line-scanning, using fewer optical components, inexpensive linear-array detectors and custom electronics, may enable smaller, simpler and lower-cost confocal microscopes. A line is formed using a cylindrical lens and scanned through the back focal plane of the objective with a galvanometric scanner. A linear CCD is used for detection. Two pupil configurations were compared for performance in imaging human tissue. In the full-pupil configuration, illumination and detection is made through the full objective pupil. In the divided pupil approach, half the pupil is illuminated and the other half is used for detection. The divided pupil configuration loses spatial and axial resolution due to a diminished NA, but the sectioning capability and rejection of background is improved. Imaging in skin and oral mucosa illustrate the performance of the two configurations. © 2012 SPIE. |
| Keywords: | linescanning; detectors; scanning; basal cell carcinoma; confocal microscopy; biopsy; skin; in-vivo; confocal microscopes; human skin; human tissues; in-vivo imaging; optical sectioning; dermatology; tissue; intra-operative; in vivo imaging; cylindrical lens; skin lesion; line-scanning microscopy; oral mucosa; linear-array; optical components; speckle; divided pupil; clinical settings; theta microscopy; alternative approach; axial resolutions; back focal planes; cellular resolution; linear ccd; noninvasive technique |
| Journal Title | Proceedings of SPIE |
| Volume: | 8207 |
| Conference Dates: | 2012 Jan 21 |
| Conference Location: | San Francisco, CA |
| ISBN: | 0277-786X |
| Publisher: | SPIE |
| Location: | San Francisco, CA |
| Date Published: | 2012-01-01 |
| Start Page: | 82070L |
| Language: | English |
| DOI: | 10.1117/12.909586 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - Progress in Biomedical Optics and Imaging - Proceedings of SPIE - Progr. Biomed. Opt. Imaging Proc. SPIE - "Conference code: 89706" - "Export Date: 4 June 2012" - "Sponsors: The Society of Photo-Optical Instrumentation Engineers (SPIE)" - 21 January 2012 through 24 January 2012 - "Source: Scopus" |
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32Abeytunge
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