Background and speckle suppression with a divided pupil and Nomarski prism for reflectance line-scanning confocal microscopy of human tissues Conference Paper
| Authors: | Glazowski, C.; Zavislan, J.; Rajadhyaksha, M. |
| Title: | Background and speckle suppression with a divided pupil and Nomarski prism for reflectance line-scanning confocal microscopy of human tissues |
| Conference Title: | Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIX |
| Abstract: | We present a design of a line-scanning confocal microscope for reduced speckle and improved sectioning performance by the use of two pupil-modification techniques. The first is a divided-pupil configuration in which the illumination and detection paths are separate in object space except in the focal (optical sectioning) plane. The second technique is a novel implementation of a Nomarski prism and quarter-wave retarder, termed NRDIC, which has shown good results in point-scanning confocal microscopy and we will present its translation to line-scanning confocal microscopy. A stable turbid phantom that simulates the background-driven speckle was used for quantitative characterization. Compared to standard full pupil line-scanning, we show improvements in signal to background of 1.8 and 9 for NRDIC and divided pupil, respectively. Preliminary imaging in human skin in-vivo demonstrates the improvements in contrast and reduction of speckle for both the NRDIC and divided pupil modes. © 2012 Copyright Society of Photo-Optical Instrumentation Engineers (SPIE). |
| Keywords: | confocal; microscopy; line-scanning; speckle; divided pupil; nrdic; turbid |
| Journal Title | Proceedings of SPIE |
| Volume: | 8227 |
| Conference Dates: | 2012 Jan 24-26 |
| Conference Location: | San Francisco, CA |
| ISBN: | 0277-786X |
| Publisher: | SPIE |
| Date Published: | 2012-01-01 |
| Start Page: | 82270Z |
| Language: | English |
| DOI: | 10.1117/12.908270 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - Progress in Biomedical Optics and Imaging - Proceedings of SPIE - Progr. Biomed. Opt. Imaging Proc. SPIE - "Conference code: 89317" - "Export Date: 1 May 2012" - 24 January 2012 through 26 January 2012 - "Source: Scopus" - Article number 82270Z |
