Reflectance confocal microscope for imaging oral tissues in vivo, potentially with line scanning as a low-cost approach for clinical use Conference Paper
| Authors: | Gary, P.; Abeytunge, S.; Zachary, E.; Milind, R. |
| Title: | Reflectance confocal microscope for imaging oral tissues in vivo, potentially with line scanning as a low-cost approach for clinical use |
| Conference Title: | Photonic Therapeutics and Diagnostics VIII |
| Abstract: | Reflectance confocal microscopy with a line scanning approach potentially offers a smaller, simpler and less expensive approach than traditional methods of point scanning for imaging in living tissues. With one moving mechanical element (galvanometric scanner), a linear array detector and off-the-shelf optics, we designed a compact (102x102x76mm) line scanning confocal reflectance microscope (LSCRM) for imaging human tissues in vivo in a clinical setting. Customdesigned electronics, based on field programmable gate array (FPGA) logic has been developed. With 405 nm illumination and a custom objective lens of numerical aperture 0.5, lateral resolution was measured to be 0.8 um (calculated 0.64 um). The calculated optical sectioning is 3.2 um. Preliminary imaging shows nuclear and cellular detail in human skin and oral epithelium in vivo. Blood flow is also visualized in the deeper connective tissue (lamina propria) in oral mucosa. Since a line is confocal only in one dimension (parallel) but not in the other, the detection is more sensitive to multiply scattered out of focus background noise than in the traditional point scanning configuration. Based on the results of our translational studies thus far, a simpler, smaller and lower-cost approach based on a LSCRM appears to be promising for clinical imaging. © 2012 SPIE. |
| Keywords: | confocal; fpga; linescanning; field programmable gate arrays (fpga); scanning; confocal microscopy; histology; medical imaging; collagen; in-vivo; blood flow; reflectance; confocal microscopes; human skin; human tissues; linear arrays; reflectance confocal microscopies; reflection; lateral resolution; numerical aperture; optical sectioning; tissue; oral; musculoskeletal system; confocal reflectance microscope; oral mucosa; background noise; lamina propria; clinical use; line scanning; clinical settings; connective tissues; living tissues; mechanical elements; objective lens; one dimension; oral tissues; out-of-focus |
| Journal Title | Proceedings of SPIE |
| Volume: | 8207 |
| Conference Dates: | 2012 Jan 21-24 |
| Conference Location: | San Francisco, CA |
| ISBN: | 0277-786X |
| Publisher: | SPIE |
| Location: | San Francisco, CA |
| Date Published: | 2012-01-01 |
| Start Page: | 82071V |
| Language: | English |
| DOI: | 10.1117/12.908374 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | --- - Progress in Biomedical Optics and Imaging - Proceedings of SPIE - Progr. Biomed. Opt. Imaging Proc. SPIE - "Conference code: 89706" - "Export Date: 4 June 2012" - "Sponsors: The Society of Photo-Optical Instrumentation Engineers (SPIE)" - 21 January 2012 through 24 January 2012 - "Source: Scopus" |
