Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels Journal Article

   


Authors: Pu, Y.; Xue, J.; Wang, W.; Xu, B.; Gu, Y.; Tang, R.; Ackerstaff, E.; Koutcher, J. A.; Achilefu, S.; Alfano, R. R.
Article Title: Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels
Abstract: The spectral changes of native fluorophores among normal fibroblasts and cancer cell lines of different metastatic ability are investigated by fluorescence spectroscopy. The normal (fibroblast), moderately metastatic (DU-145), and advanced metastatic (PC-3) cell lines were each selectively excited at 300 nm, and their fluorescence emission spectra are analyzed using principal component analysis to explore the differences of the relative contents of tryptophan and reduced nicotinamide adenine dinucleotide in these cell lines. The results show that the tryptophan emission featured predominantly in the fluorescence spectra of the advanced metastatic cancer cells in comparison with the moderately metastatic cancer and normal cells. © 2013 Society of Photo-Optical Instrumentation Engineers.
Keywords: tryptophan; nadh; normal; moderately metastatic and advanced metastatic cell lines; optical biopsy; metastasisrelevant key fluorophores; native fluorescence spectra
Journal Title: Journal of Biomedical Optics
Volume: 18
Issue: 8
ISSN: 1083-3668
Publisher: SPIE  
Date Published: 2013-08-01
Start Page: 87002
Language: English
DOI: 10.1117/1.jbo.18.8.087002
PROVIDER: scopus
PMCID: PMC3763931
PUBMED: 23912761
DOI/URL:

http://www.scopus.com/inward/record.url?eid=2-s2.0-84892537219&partnerID=40&md5=471ac13228ee186287b349b7072af404
Notes: Export Date: 3 March 2014 -- CODEN: JBOPF -- Source: Scopus
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MSK Authors
  1. Jason A Koutcher
    278 Koutcher
  2. Ellen Ackerstaff
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