Development of in vitro models to elucidate mechanisms of intrinsic cellular and tissue fluorescence Conference Paper
| Authors: | Savage, H. E.; Kolli, V.; Saha, S.; Zhang, J. C.; Glasgold, M.; Sacks, P. G.; Alfano, R. R.; Schantz, S. P. |
| Editors: | Alfano, R. R.; Katzir, A. |
| Title: | Development of in vitro models to elucidate mechanisms of intrinsic cellular and tissue fluorescence |
| Conference Title: | Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II |
| Abstract: | In vitro cell model systems have been used to study the mechanisms of intrinsic cellular and tissue fluorescence as a potential biomarker for cancer. Phenotypic characteristics of cancer that are different from normal tissue include changes in histoarchitecture, proliferation rates and differentiation. A nitrosmethlybenzylamine (NMBA)/rat esophageal carcinogenesis model (NMBA) , a transforming growth factor beta (TGF-β)/normal epithelial cell model, and a retinoic acid (RA)/multicellular tumor spheroid inodel(RAMTS) were used to assess fluorescence changes associated respectively with changes in histoarchitecture, proliferation rates and differentiation. A xenon based fluorescence spectrophotometer (Mediscience Corp.) was used to collect excitation and emission spectra. Two excitation scans (λEx 200-'360nm, AEm 380nm; )Ex 240-430nm, ).Em 450nm) and two emission scans (λEx 300nm, )Em 320-580nm; )Ex 34Ornn, AEm 360-660nm) were used to analyze the three model systems. Using the NMBA model, differences were seen in the excitation scan λEx 200-360nm, )Em 38Orim) and the emission scan ()λEx 340nm, ).Em 360-660nm) when normal rat esophageal tissue was compared to hyperplastic and tumor tissue. In the (TGF-) model, differences were seen in the excitation scan (Ex 240-430nm, AEm 450nm) when comparing proliferation slowed (TGF-treated) epithelial cells to their untreated controls. In the RAMTS model, differences were seen with all four scans when RA treated multicellular tumor spheroids (non-'differentiating) were compared to untreated control cells (differentiating). The data indicate that fluorescence changes seen in these model systems may relate to changes in histoarchitecture, proliferation rates and differentiation. Their relationship to in vivo fluorescence changes seen in cancer patients remains to be elucidated. © 1995 SPIE. All rights reserved. |
| Keywords: | cell proliferation; cytology; transforming growth factor beta; fluorescence; cell differentiation; tumors; tissue engineering; diseases; model system; cells; excitation and emission spectra; emission spectroscopy; multicellular tumor spheroids; cellular fluorescence; fluorescence model systems; cell histoarchitecture; carcinogenesis models; esophageal tissues; fluorescence spectrophotometer |
| Journal Title | Proceedings of Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases II |
| Volume: | 2387 |
| Conference Dates: | 1995 Feb 7-8 |
| Conference Location: | San Jose, CA |
| ISBN: | 0-8194-1734-3 |
| Publisher: | SPIE |
| Date Published: | 1995-01-01 |
| Start Page: | 44 |
| End Page: | 56 |
| Language: | English |
| DOI: | 10.1117/12.206835 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Source: Scopus |
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