Native cellular fluorescence identifies terminal squamous differentiation of normal oral epithelial cells in culture: A potential chemoprevention biomarker Journal Article
| Authors: | Sacks, P. G.; Savage, H. E.; Levine, J.; Kolli, V. R.; Alfano, R. R.; Schantz, S. P. |
| Article Title: | Native cellular fluorescence identifies terminal squamous differentiation of normal oral epithelial cells in culture: A potential chemoprevention biomarker |
| Abstract: | Native cellular fluorescence (NCF) is being investigated as an intermediate endpoint biomarker for chemoprevention. Oral epithelial cells were cultured under three conditions to identify a spectral pattern for epithelial differentiation: cells maintained in serum-free keratinocyte growth medium were the least differentiated (KGM cells); cells switched to DMEM/F12 plus 10% FCS were intermediate in differentiation (DMEM/F12/FCS cells); DMEM/F12/FCS cells switched to serum-free DMEM/F12 plus 0.8 M NaCl to induce cornified envelopes were the most differentiated (DMEM/F12/NaCl cells). The differentiation status was characterized using immunohistochemistry and electron microscopy. NCF analysis was able to distinguish terminally differentiated epithelial cells (DMEM/F12/NaCl) from those less differentiated cells (KGM, DMEM/F12/FCS) in several emission (λ(ex) 340 nm, λ(em), 360-660 nm; λ; 365 nm, λ, 400-700 nm; λ(em) 420 nm, λ(em) 440-800 nm) and excitation scans (λ(em) 200-360 nm; λ(em) 380 nm, λ(em) 240-430 nm; λ(em) 450 nm,; λ 250-460 nm, λ 480 nm; λ 270-500 nm, λ 520 nm). The ability to discriminate terminal differentiation in this in vitro model supports the concept of using NCF as an intermediate biomarker to monitor in vivo mucosal differentiation. |
| Keywords: | immunohistochemistry; human tissue; human cell; electron microscopy; chemoprophylaxis; cell differentiation; cell culture; biomarker; fluorescence spectroscopy; cell marker; mouth mucosa; fluorescence analysis; differentiation; chemoprevention; squamous epithelium; human; priority journal; article; oral epithelial cells |
| Journal Title: | Cancer Letters |
| Volume: | 104 |
| Issue: | 2 |
| ISSN: | 0304-3835 |
| Publisher: | Elsevier Ireland Ltd. |
| Date Published: | 1996-07-12 |
| Start Page: | 171 |
| End Page: | 181 |
| Language: | English |
| DOI: | 10.1016/0304-3835(96)04246-2 |
| PUBMED: | 8665485 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 22 November 2017 -- Source: Scopus |
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