Investigation of native fluorescence spectral difference among prostate cancer cell lines with different risk levels Conference Paper

  

Authors:	Pu, Y.; Xue, J.; Xu, B.; Wang, W.; Gu, Y.; Tang, R.; Achilefu, S.; Ackerstaff, E.; Koutcher, J. A.; Alfano, R. R.
Title:	Investigation of native fluorescence spectral difference among prostate cancer cell lines with different risk levels
Conference Title:	Conference on Optical Biopsy XI
Abstract:	The alteration of native fluorophores among different types of cancer cell lines was investigated by the fluorescence spectroscopy. Different types of cancer cell lines with different risk levels, such as moderate metastatic (DU-145) and advanced metastatic (PC-3) cell lines as well as normal cell line (Fibroblast), were excited by the selective excitation wavelength of 300 nm to explore changes of the relative contents of tryptophan and NADH using principal component analysis (PCA). The higher relative content of tryptophan was observed in the advanced metastatic cancer cell lines in comparison with the moderate metastatic and non aggressive cell lines. © 2013 Copyright SPIE.
Keywords:	fluorescence; biopsy; cell culture; fibroblast; fibroblasts; fluorescence spectroscopy; amino acids; principal component analysis; native fluorescence; du-145; pc-3; prostate cancer cells; cancer cell lines with different risk levels; metastatic cancer cells; risk levels; selective excitations; spectral differences
Journal Title	Proceedings of SPIE
Volume:	8577
Conference Dates:	2013 Feb 5-6
Conference Location:	San Francisco, CA
ISBN:	0277-786X
Publisher:	SPIE
Location:	San Francisco, CA
Date Published:	2013-01-01
Start Page:	85770X
Language:	English
PROVIDER:	scopus
DOI:	10.1117/12.2001443
DOI/URL:	http://www.scopus.com/inward/record.url?eid=2-s2.0-84878495960&partnerID=40&md5=398b3fcd72c15192460af0bc5872d7a8
Notes:	--- - Progress in Biomedical Optics and Imaging - Proceedings of SPIE - Progr. Biomed. Opt. Imaging Proc. SPIE - "Conference code: 97257" - "Export Date: 1 July 2013" - ":doi 10.1117/12.2001443" - "Sponsors: The Society of Photo-Optical Instrumentation Engineers (SPIE); Association of Italian American Educators (AIAE); BaySpec, Inc.; Hamamatsu Corporation; MagneGas Corporation" - 5 February 2013 through 6 February 2013 - "Source: Scopus"

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