Native fluorescence spectroscopic evaluation of chemotherapeutic effects on malignant cells using nonnegative matrix factorization analysis Journal Article
| Authors: | Pu, Y.; Tang, G. C.; Wang, W. B.; Savage, H. E.; Schantz, S. P.; Alfano, R. R. |
| Article Title: | Native fluorescence spectroscopic evaluation of chemotherapeutic effects on malignant cells using nonnegative matrix factorization analysis |
| Abstract: | The native fluorescence spectra of retinoic acid (RA)-treated and untreated human breast cancerous cells excited with the selective wavelengths of 300 nm and 340 nm were measured and analyzed using a blind source separation method namely Nonnegative Matrix Factorization (NMF). The results show that the fluorophores of human malignant breast cells change their compositions when they are treated with RA. The reduced contribution from tryptophan, NADH and flavin to the fluorescence of the treated breast cancerous cells was observed in comparison with that of the untreated cells. The results indicate that the decrease of adenosine triphosphate (ATP) in the RA-treated cells. The possible clinical applications of this native fluorescence study are discussed. ©Adenine Press (2011). |
| Keywords: | cancer chemotherapy; controlled study; human cell; comparative study; breast cancer; fluorescence; antineoplastic activity; quercetin; cancer cell; data analysis; collagen; fluorescence spectroscopy; adenosine triphosphate; retinoic acid; nicotinamide adenine dinucleotide; tryptophan; elastin; chemical composition; breast cancer cells; chemo-therapeutic effect; flavin; native fluorescence; nonnegative matrix factorization (nmf); reduced nicotinamide adenine dinucleotide (nadh); selective excitation wavelength; nitrate reductase (nadh); nonnegative matrix factorization; waveform |
| Journal Title: | Technology in Cancer Research & Treatment |
| Volume: | 10 |
| Issue: | 2 |
| ISSN: | 1533-0346 |
| Publisher: | Sage Publications, Inc. |
| Date Published: | 2011-04-01 |
| Start Page: | 113 |
| End Page: | 120 |
| Language: | English |
| PROVIDER: | scopus |
| PUBMED: | 21381789 |
| DOI/URL: | |
| Notes: | --- - "Cited By (since 1996): 1" - "Export Date: 23 June 2011" - "CODEN: TCRTB" - "Source: Scopus" |
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