Retinoic acid causes a decline in TGF-α expression, cloning efficiency, and tumorigenicity in a human embryonal cancer cell line Journal Article
| Authors: | Dmitrovsky, E.; Moy, D.; Miller, W. H. Jr; Li, A.; Masui, H. |
| Article Title: | Retinoic acid causes a decline in TGF-α expression, cloning efficiency, and tumorigenicity in a human embryonal cancer cell line |
| Abstract: | The human teratocarcinoma NTERA-2 cl. D1 (NT2/D1) cell is a cloned multipotential embryonal cancer cell line that differentiates into a neuronal phenotype and other cellular lineages with retinoic acid (RA) treatment. Here we report that mRNA for the transforming frowth factor-α is expressed in these RA-untreated cells and that RA-treatment results in a reduction of mRNA expression within 24 hr of treatment. In total cellular RNA, TGF-α mRNA is not detectable by Northern analysis at 6 days when there is increased expression of the human homeotic genes Hu-1 (Hox 2.1) and Hu-2 (Hox 2.2), known markers of Ra response in NT2/D1 cells. RA treatment also causes a marked reduction in bloning efficiency and tumorigenicity of these cells. The addition of TGF-α or EGF (epidermal growth factor) protein to RA-untreated NT2/D1 cells augments soft agar cloning under limited fetal calf serum conditions. Blocking monoclonal antibodies directed against the EGF receptor (EGFr) can prevent this augmentation. We conclude that TGF-α expression inversely correlates with the state of RA-induced differentiation of this human teratocarcinoma cell and that TGF-α and EGF proteins are stimulatory growth factors in NT2/D1 cells under these culture conditions. |
| Keywords: | epidermal growth factor; human cell; gene expression; cell line; cell differentiation; transcription, genetic; tumor cells, cultured; kinetics; cell culture; messenger rna; rna, messenger; tumor cell line; carcinogenicity; transforming growth factors; teratoma; retinoic acid; clone cells; embryonal carcinoma; differentiation; transforming growth factor alpha; tretinoin; human; priority journal; article; support, non-u.s. gov't; human teratocarcinoma; tranforming growth factor α |
| Journal Title: | Oncogene Research |
| Volume: | 5 |
| Issue: | 3 |
| ISSN: | 0890-6487 |
| Publisher: | Harwood Academic Publishers |
| Date Published: | 1990-01-01 |
| Start Page: | 233 |
| End Page: | 239 |
| Language: | English |
| PUBMED: | 2320376 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Article -- Export Date: 27 January 2020 -- Source: Scopus |
