Retrogenic color-barcoding for fate mapping of single innate lymphocytes Journal Article
| Authors: | Grassmann, S.; Sun, J. C.; Buchholz, V. R. |
| Article Title: | Retrogenic color-barcoding for fate mapping of single innate lymphocytes |
| Abstract: | Lymphocyte fate mapping using single-cell transfers has been used to study T and B cell differentiation. Recently, retrogenic color-barcoding has allowed the extension of this approach to single innate lymphocytes such as NK cells. This new and versatile technology is based on the transduction of hematopoietic stem cells (HSCs) with a collection of retroviruses encoding distinct fluorescent proteins. Through combinatorial transduction, fluorescent protein barcodes are generated, which are inherited by the progeny of HSCs after transfer. By sorting individual cells expressing unique color-barcodes from the mature lymphocyte populations derived from these HSCs, it is now possible to track the fate of innate lymphocytes in vivo. © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature. |
| Keywords: | cell differentiation; hematopoietic stem cells; natural killer cell; killer cells, natural; hematopoiesis; virus infection; hematopoietic stem cell; nk cells; single-cell fate mapping; color-barcoding; retrogenic mice |
| Journal Title: | Methods in Molecular Biology |
| Volume: | 2463 |
| ISSN: | 1064-3745 |
| Publisher: | Humana Press Inc |
| Date Published: | 2022-01-01 |
| Start Page: | 117 |
| End Page: | 127 |
| Language: | English |
| DOI: | 10.1007/978-1-0716-2160-8_9 |
| PUBMED: | 35344171 |
| PROVIDER: | scopus |
| DOI/URL: | |
| Notes: | Book Chapter 9 in "Natural Killer (NK) Cells: Methods and Protocols" (ISBN: 978-1-0716-2159-2) -- Export Date: 2 May 2022 -- Source: Scopus |
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