Assessing lineage and cytolytic functional potential of murine tissue-resident innate lymphocytes Journal Article
| Authors: | Nixon, B. G.; Chou, C.; Li, M. O. |
| Article Title: | Assessing lineage and cytolytic functional potential of murine tissue-resident innate lymphocytes |
| Abstract: | Group 1 innate lymphocytes are heterogeneous, and their ontogeny and function remain ambiguous. Here, we describe a protocol to measure cell ontogeny and effector functions of natural killer (NK) and ILC1 subsets based on current understanding of their differentiation pathways. We use cre drivers to genetically fate-map cells, tracking plasticity between mature NK and ILC1. We describe innate lymphoid cell precursor transfer studies that determine ontogeny of granzyme-C-expressing ILC1. Additionally, we detail in vitro killing assays that test cytolytic potential of ILC1s. For complete details on the use and execution of this protocol, please refer to Nixon et al. (2022).1 © 2023 The Author(s) |
| Keywords: | genetics; immunology; cell isolation; cell-based assays; model organisms; flow cytometry/mass cytometry |
| Journal Title: | STAR Protocols |
| Volume: | 4 |
| Issue: | 1 |
| ISSN: | 2666-1667 |
| Publisher: | Cell Press |
| Date Published: | 2023-03-17 |
| Start Page: | 102136 |
| Language: | English |
| DOI: | 10.1016/j.xpro.2023.102136 |
| PROVIDER: | scopus |
| PMCID: | PMC10006489 |
| PUBMED: | 36861825 |
| DOI/URL: | |
| Notes: | Article -- MSK Cancer Center Support Grant (P30 CA008740) acknowledged in PDF -- MSK corresponding author is Ming Li -- Source: Scopus |
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