Purification and biochemical characterization of human pluripotent hematopoietic colony-stimulating factor Journal Article

   


Authors: Welte, K.; Platzer, E.; Lu, L.; Gabrilove, J. L.; Levi, E.; Mertelsmann, R.; Moore, M. A. S.
Article Title: Purification and biochemical characterization of human pluripotent hematopoietic colony-stimulating factor
Abstract: Pluripotent hematopoietic colony-stimulating factor (pluripotent CSF), a protein that is constitutively produced by the human bladder carcinoma cell line 5637, has been purified from low serum (0.2% fetal calf serum)-containing conditioned medium. The purification involved sequential ammonium sulfate precipitation, ion-exchange chromatography, gel filtration, and reversed-phase high-performance liquid chromatography. The purified protein has a molecular weight of 18,000 in NaDodSO4/polyacrylamide gel electrophoresis, both by the silver staining technique and by elution of biological activity from a corresponding gel slice, and has an isoelectric point of 5.5. Pluripotent CSF supports the growth of human mixed colonies, granulocyte-macrophage colonies, and early erythroid colonies and induces differentiation of the human promyelocytic leukemic cell line HL-60 and the murine myelomonocytic leukemic cell line WEHI-3B (D+). The specific activity of the purified pluripotent CSF in the granulocyte-macrophage colony assay is 1.5 x 108 units/mg of protein.
Keywords: human cell; cell line; urinary bladder neoplasms; protein purification; hematopoietic cell; hematopoietic stem cells; murinae; hematopoiesis; biological assay; bladder carcinoma; multipotent stem cell; molecular weight; colony-stimulating factors; ion exchange chromatography; culture media; bladder; drug purification; granulocyte precursor; colony stimulating factor; humans; human; priority journal; article; isoelectric point; burst forming unit; blood and hemopoietic system
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 82
Issue: 5
ISSN: 0027-8424
Publisher: National Academy of Sciences  
Date Published: 1985-03-01
Start Page: 1526
End Page: 1530
Language: English
DOI: 10.1073/pnas.82.5.1526
PROVIDER: scopus
PMCID: PMC397296
PUBMED: 3871951
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0001004485&doi=10.1073%2fpnas.82.5.1526&partnerID=40&md5=c60635b829bd3a61bd1f90b628acb9f2
Notes: Article -- Export Date: 26 October 2021 -- Source: Scopus
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MSK Authors
  1. Malcolm A S Moore
    549 Moore
  2. Janice Gabrilove
    122 Gabrilove
  3. Ester Levi
    17 Levi
  4. Karl Welte
    77 Welte
  5. Roland Mertelsmann
    32 Mertelsmann
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