Authors: | Welte, K.; Platzer, E.; Lu, L.; Gabrilove, J. L.; Levi, E.; Mertelsmann, R.; Moore, M. A. S. |
Article Title: | Purification and biochemical characterization of human pluripotent hematopoietic colony-stimulating factor |
Abstract: | Pluripotent hematopoietic colony-stimulating factor (pluripotent CSF), a protein that is constitutively produced by the human bladder carcinoma cell line 5637, has been purified from low serum (0.2% fetal calf serum)-containing conditioned medium. The purification involved sequential ammonium sulfate precipitation, ion-exchange chromatography, gel filtration, and reversed-phase high-performance liquid chromatography. The purified protein has a molecular weight of 18,000 in NaDodSO4/polyacrylamide gel electrophoresis, both by the silver staining technique and by elution of biological activity from a corresponding gel slice, and has an isoelectric point of 5.5. Pluripotent CSF supports the growth of human mixed colonies, granulocyte-macrophage colonies, and early erythroid colonies and induces differentiation of the human promyelocytic leukemic cell line HL-60 and the murine myelomonocytic leukemic cell line WEHI-3B (D+). The specific activity of the purified pluripotent CSF in the granulocyte-macrophage colony assay is 1.5 x 108 units/mg of protein. |
Keywords: | human cell; cell line; urinary bladder neoplasms; protein purification; hematopoietic cell; hematopoietic stem cells; murinae; hematopoiesis; biological assay; bladder carcinoma; multipotent stem cell; molecular weight; colony-stimulating factors; ion exchange chromatography; culture media; bladder; drug purification; granulocyte precursor; colony stimulating factor; humans; human; priority journal; article; isoelectric point; burst forming unit; blood and hemopoietic system |
Journal Title: | Proceedings of the National Academy of Sciences of the United States of America |
Volume: | 82 |
Issue: | 5 |
ISSN: | 0027-8424 |
Publisher: | National Academy of Sciences |
Date Published: | 1985-03-01 |
Start Page: | 1526 |
End Page: | 1530 |
Language: | English |
DOI: | 10.1073/pnas.82.5.1526 |
PROVIDER: | scopus |
PMCID: | PMC397296 |
PUBMED: | 3871951 |
DOI/URL: | |
Notes: | Article -- Export Date: 26 October 2021 -- Source: Scopus |