Recombinant human granulocyte colony-stimulating factor: Effects on normal and leukemic myeloid cells Journal Article

  


Authors: Souza, L. M.; Boone, T. C.; Gabrilove, J.; Lai, P. H.; Zsebo, K. M.; Murdock, D. C.; Chazin, V. R.; Bruszewski, J.; Lu, H.; Chen, K. K.; Barendt, J.; Platzer, E.; Moore, M. A. S.; Mertelsmann, R.; Welte, K.
Article Title: Recombinant human granulocyte colony-stimulating factor: Effects on normal and leukemic myeloid cells
Abstract: Experiments were conducted to isolate and characterize the gene and gene product of a human hematopoietic colony-stimulating factor with pluripotent biological activities. This factor has the ability to induce differentiation of a murine myelomonocytic leukemia cell line WEHI-3B(D+) and cells from patients with newly diagnosed acute nonlymphocytic leukemia (ANLL). A complementary DNA copy of the gene encoding a pluripotent human granulocyte colony-stimulating factor (hG-CSF) was cloned and expressed in Escherichia. colt. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay. In addition, recombinant hG-CSF can support early erythroid colonies and mixed colony formation. Competitive binding studies done with 125I-labeled hG-CSF and cell samples from two patients with newly diagnosed human leukemias as well as WEHI-3B(D+) cells showed that one of the human leukemias (ANLL, classified as M4) and the WEHI-3B(D+) cells have receptors for hG-CSF. Furthermore, the murine WEHI-3B(D+) cells and human leukemic cells classified as M2, M3, and M4 were induced by recombinant hG-CSF to undergo terminal differentiation to macrophages and granulocytes. The secreted form of the protein produced by the bladder carcinoma cell line 5637 was found to be O-glycosylated and to have a molecular weight of 19,600.
Keywords: leukemia; unclassified drug; drug efficacy; animals; mice; heredity; genes; cell line; cell differentiation; in vitro study; molecular cloning; dna; genetic engineering; leukemia, myeloid; escherichia coli; recombinant proteins; murinae; plasmids; bone marrow cell; radioisotope; colony-forming units assay; granulocyte colony stimulating factor; granulocyte colony-stimulating factor; adverse drug reaction; colony-stimulating factors; reticuloendothelial system; drug comparison; gene isolation; granulocytes; topical drug administration; humans; human; priority journal; article; nonlymphatic leukemia; blood and hemopoietic system; colony stimulating factor i 125
Journal Title: Science
Volume: 232
Issue: 4746
ISSN: 0036-8075
Publisher: American Association for the Advancement of Science  
Date Published: 1986-04-04
Start Page: 61
End Page: 65
Language: English
DOI: 10.1126/science.2420009
PUBMED: 2420009
PROVIDER: scopus
DOI/URL:

https://www.scopus.com/inward/record.uri?eid=2-s2.0-0022549329&doi=10.1126%2fscience.2420009&partnerID=40&md5=b89028e50927c7695704c77e70834571
Notes: Article -- Export Date: 18 August 2021 -- Source: Scopus
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MSK Authors
  1. Malcolm A S Moore
    549 Moore
  2. Janice Gabrilove
    122 Gabrilove
  3. Karl Welte
    77 Welte
  4. Roland Mertelsmann
    32 Mertelsmann
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